Sparse force‑bearing bridges between neighboring synaptic vesicles
Keywords: 
RRP
Reserve
Supply-rate depression
Presynaptic
Tomography
Issue Date: 
2019
Publisher: 
Springer Science and Business Media LLC
ISSN: 
1863-2653
Note: 
his article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Citation: 
Wesseling, J.F. (John F.); Phan, S. (Sébastien); Bushong, E.A. (Eric A.); et al. "Sparse force‑bearing bridges between neighboring synaptic vesicles". Brain Structure and Function. 224, 2019, 3263 - 3276
Abstract
Most vesicles in the interior of synaptic terminals are clustered in clouds close to active zone regions of the plasma membrane where exocytosis occurs. Electron-dense structures, termed bridges, have been reported between a small minority of pairs of neighboring vesicles within the clouds. Synapsin proteins have been implicated previously, but the existence of the bridges as stable structures in vivo has been questioned. Here we use electron tomography to show that the bridges are present but less frequent in synapsin knockouts compared to wildtype. An analysis of distances between neighbors in wildtype tomograms indicated that the bridges are strong enough to resist centrifugal forces likely induced by fxation with aldehydes. The results confrm that the bridges are stable structures and that synapsin proteins are involved in formation or stabilization.

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