C. elegans DPPH scavenging Green tea Rosmarinic acid
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Torre, M.P. (María Pilar) de; Cavero-Remon, R.Y. (Rita Yolanda); Calvo, M.I. (María Isabel); et al. "A simple and a reliable method to quantify antioxidant activity in vivo". Antioxidants. 8 (5), 2019, 142
The characterization of compounds with antioxidant activity is of great interest due to
their ability to reduce reactive oxygen species production and, therefore, prevent some age-related
diseases. Its antioxidant capacity can be analyzed by different methods both in vitro and in vivo.
Caenorhabditis elegans is an in vivo model widely used in ageing research. Until now, available tests
analyze functional effects in the worms, so the antioxidant activity of the compound is indirectly
monitored. We have developed a simple and a reliable method to quantify internal antioxidant
activity in vivo. To validate this method, we analyzed an aqueous green tea extract and two other
compounds with a well-known antioxidant activity and without this activity. The results obtained
(EC50 green tea = 21.76 ± 1.28 µg/mL; EC50 positive control = 8.50 ± 0.33 µg/mL; negative control
EC50 > 500 µg/mL) can help in the design of further in vivo experiments. Thus, our method can be
used as a previous screening capable of reducing the gap between in vitro and in vivo assays.