In vivo study of the bioavailability and metabolic profile of (poly)phenols after sous-vide artichoke consumption
Keywords: 
Globe artichoke
(Poly)phenols
Caffeoylquinic acids
Metabolism
Bioavailability
UHPLC-MS/MS
COMT methylation
Issue Date: 
2022
Publisher: 
Elsevier
ISSN: 
0308-8146
Note: 
This is an open access article under the CC BY-NC-ND license
Citation: 
Dominguez-Fernandez, M. (Maite); Tie-Yang, P. Y. (Paul Yong); Ludwig, I. A. (Iziar Amaia); et al. "In vivo study of the bioavailability and metabolic profile of (poly)phenols after sous-vide artichoke consumption". Food Chemistry. (367), 2022, 130620
Abstract
Artichokes are a rich source of (poly)phenols, mainly caffeoylquinic acids, but little is known about their bioavailability from this source. This study investigated the absorption, metabolism and excretion of (poly)phenols after sous-vide artichoke consumption (5776 µmol of (poly)phenols) by healthy volunteers. Seventy-six (poly)phenol metabolites were identified by UHPLC-MS/MS using authentic standards, including acyl-quinic acids plus C6–C3, C6–C1, C6–C2, C6–C1–N, C6–C0 metabolites, and their phase-II conjugates. The major metabolites were 3ʹ-methoxy-4ʹ-hydroxycinnamic acid, 3ʹ-methoxycinnamic acid-4ʹ-sulfate, and 4ʹ-hydroxycinnamic acid-3ʹ-sulfate, which appeared early in plasma (Tmax < 4 h); plus 3-(3ʹ-methoxy-4ʹ-hydroxyphenyl)propanoic acid, 3-(4ʹ-methoxyphenyl)propanoic acid-3ʹ-glucuronide, 3-(3ʹ-hydroxyphenyl) propanoic acid and hippuric acids, which appeared later (Tmax > 6 h). The 24 h urinary recovery averaged 8.9% (molar basis) of the (poly)phenols consumed. Hepatic beta-oxidation of 3ʹ,4ʹ-dihydroxycinnamic acid and methylated conjugates occurred, but was limited (<0.04%). 3ʹ-Methylation exceeded 4ʹ-methylation and interindividual variability was high, especially for gut microbial metabolites (up to 168-fold).

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