Danio rerio as model organism for adenoviral vector evaluation
Keywords: 
Danio rerio
Adenovirus
High-capacity adenoviral vector
Helper-dependent adenovirus
Delivery system
Gene therapy
Issue Date: 
2019
Publisher: 
MDPI AG
ISSN: 
2073-4425
Note: 
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Citation: 
Gulías, P. (Paola); Guerra-Varela, J. (Jorge); Gonzalez-Aparicio, M. (Manuela); et al. "Danio rerio as model organism for adenoviral vector evaluation". Genes. 10 (12), 2019, 1053
Abstract
Viral vector use is wide-spread in the field of gene therapy, with new clinical trials starting every year for different human pathologies and a growing number of agents being approved by regulatory agencies. However, preclinical testing is long and expensive, especially during the early stages of development. Nowadays, the model organism par excellence is the mouse (Mus musculus), and there are few investigations in which alternative models are used. Here, we assess the possibility of using zebrafish (Danio rerio) as an in vivo model for adenoviral vectors. We describe how E1/E3-deleted adenoviral vectors achieve efficient transduction when they are administered to zebrafish embryos via intracranial injection. In addition, helper-dependent (high-capacity) adenoviral vectors allow sustained transgene expression in this organism. Taking into account the wide repertoire of genetically modified zebrafish lines, the ethical aspects, and the affordability of this model, we conclude that zebrafish could be an efficient alternative for the early-stage preclinical evaluation of adenoviral vectors.

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