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dc.creatorGulías, P. (Paola)-
dc.creatorGuerra-Varela, J. (Jorge)-
dc.creatorGonzalez-Aparicio, M. (Manuela)-
dc.creatorRicobaraza, A. (Ana)-
dc.creatorVales, A. (África)-
dc.creatorGonzález-Aseguinolaza, G. (Gloria)-
dc.creatorHernandez-Alcoceba, R. (Rubén)-
dc.creatorSánchez, L. (Laura)-
dc.date.accessioned2022-05-24T06:55:35Z-
dc.date.available2022-05-24T06:55:35Z-
dc.date.issued2019-
dc.identifier.citationGulías, P. (Paola); Guerra-Varela, J. (Jorge); Gonzalez-Aparicio, M. (Manuela); et al. "Danio rerio as model organism for adenoviral vector evaluation". Genes. 10 (12), 2019, 1053es
dc.identifier.issn2073-4425-
dc.identifier.urihttps://hdl.handle.net/10171/63512-
dc.description.abstractViral vector use is wide-spread in the field of gene therapy, with new clinical trials starting every year for different human pathologies and a growing number of agents being approved by regulatory agencies. However, preclinical testing is long and expensive, especially during the early stages of development. Nowadays, the model organism par excellence is the mouse (Mus musculus), and there are few investigations in which alternative models are used. Here, we assess the possibility of using zebrafish (Danio rerio) as an in vivo model for adenoviral vectors. We describe how E1/E3-deleted adenoviral vectors achieve efficient transduction when they are administered to zebrafish embryos via intracranial injection. In addition, helper-dependent (high-capacity) adenoviral vectors allow sustained transgene expression in this organism. Taking into account the wide repertoire of genetically modified zebrafish lines, the ethical aspects, and the affordability of this model, we conclude that zebrafish could be an efficient alternative for the early-stage preclinical evaluation of adenoviral vectors.es_ES
dc.description.sponsorshipThis researchwas funded byXunta de Galicia, Local Government, grant number ED431C 2018/28, and Gobierno de Navarra, Local Government, grant numbers 0011-1383-2018-000011 PT038 and 0011-1383-2019-000006 PT013 (XANTOGEN).es_ES
dc.language.isoenges_ES
dc.publisherMDPI AGes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectDanio rerioes_ES
dc.subjectAdenoviruses_ES
dc.subjectHigh-capacity adenoviral vectores_ES
dc.subjectHelper-dependent adenoviruses_ES
dc.subjectDelivery systemes_ES
dc.subjectGene therapyes_ES
dc.titleDanio rerio as model organism for adenoviral vector evaluationes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.noteThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).es_ES
dc.identifier.doi10.3390/genes10121053-
dadun.citation.number12es_ES
dadun.citation.publicationNameGeneses_ES
dadun.citation.startingPage1053es_ES
dadun.citation.volume10es_ES

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