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dc.creatorDieter, C. (Cristine)-
dc.creatorSilveira-Assmann, T. (Taís)-
dc.creatorRodrigues-Costa, A. (Aline)-
dc.creatorCanani, L.H. (Luís Henrique)-
dc.creatorMarmontel-de-Souza, B. (Bianca)-
dc.creatorBauer, A.C. (Andrea Carla)-
dc.creatorCrispim, D. (Daisy)-
dc.identifier.citationDieter, C. (Cristine); Silveira-Assmann, T. (Taís); Rodrigues-Costa, A. (Aline); et al. "MiR-30e-5p and MiR-15a-5p expressions in plasma and urine of type 1 diabetic patients with diabetic kidney disease". Frontiers in Genetics. 10, 2019, 563es_ES
dc.description.abstractIntroduction: Diabetic kidney disease (DKD) is a common microvascular complication that affects 40% of patients with diabetes mellitus (DM). Emerging evidence suggests a role for several microRNAs (miRNAs) in the development of DKD. In this context, miR-15a-5p and miR-30e-5p have been shown to regulate the expression of the uncoupling protein 2 (UCP2), a mitochondrial protein that decreases reactive oxygen species (ROS) formation by the mitochondria. Since ROS overproduction is a key contributor to the pathogenesis of DKD, dysregulation of these two miRNAs could be involved in DKD pathogenesis. Thus, the aim of this study was to compare the expressions of miR-15a-5p and miR-30e-5p in type 1 DM (T1DM) patients with DKD (cases) and without this complication (controls), and to perform bioinformatics analyses to investigate their putative targets and biological pathways under their regulation. Methods: MiR-15a-5p and miR-30e-5p expressions were analyzed in plasma and urine of 17 T1DM controls and 23 DKD cases (12 with moderate DKD and 11 with severe DKD) using qPCR. Bioinformatics analyses were performed in Cytoscape software. Results: MiR-30e-5p expression was downregulated in plasma of patients with moderate and severe DKD compared to T1DM controls. Moreover, this miRNA was also downregulated in urine of patients with severe DKD compared to the other groups. No difference was found in miR-15a-5p expression between groups. Bioinformatics analyses indicated that miR-30e-5p and miR-15a-5p regulate various genes that participate in pathways related to angiogenesis, apoptosis, cell differentiation, oxidative stress, and hypoxia. Conclusion: MiR-30e-5p seems to be downregulated in plasma and urine of patients with DKD.es_ES
dc.description.sponsorshipThis study was partially supported by grants from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundo de Incentivo à Pesquisa e Eventos (FIPE) at Hospital de Clínicas de Porto Alegre (Grant No. 2017-0095), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) – Finance Code 001, and PostGraduated Program in Medical Sciences: Endocrinology – Federal University of Rio Grande do Sul. DC and LC are recipients of scholarships from CNPq, while CD, TA, and BdS are recipients of scholarships from the CAPES.es_ES
dc.publisherFrontiers Media SAes_ES
dc.subjectMicroRNA expressiones_ES
dc.subjectDiabetic kidney diseasees_ES
dc.subjectBioinformatics analysises_ES
dc.subjectType 1 diabetes mellituses_ES
dc.titleMiR-30e-5p and MiR-15a-5p expressions in plasma and urine of type 1 diabetic patients with diabetic kidney diseasees_ES
dc.description.noteThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.es_ES
dadun.citation.publicationNameFrontiers in Geneticses_ES

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