Full metadata record
DC FieldValueLanguage
dc.creatorZabalza-Barangua, A. (Ana)-
dc.creatorSan-Roman, B. (Beatriz)-
dc.creatorChacon-Diaz, C. (Carlos)-
dc.creatorMiguel, M.J. (María Jesús) de-
dc.creatorMuñoz, P. (Pilar)-
dc.creatorIriarte, M. (Maite)-
dc.creatorBlasco, J.M. (J. M.)-
dc.creatorGrillo, M.J. (María Jesús)-
dc.date.accessioned2022-06-09T12:05:15Z-
dc.date.available2022-06-09T12:05:15Z-
dc.date.issued2019-
dc.identifier.citationZabalza-Barangua, A. (Ana); San-Roman, B. (Beatriz); Chacon-Diaz, C. (Carlos); et al. "GFP tagging of Brucella melitensis Rev1 allows the identification of vaccinated sheep". Transboundary and Emerging Diseases. 2019 (66), 2019, 505 - 516es
dc.identifier.issn1865-1674-
dc.identifier.urihttps://hdl.handle.net/10171/63630-
dc.description.abstractBrucellosis is a worldwide zoonosis causing important economic loss and a public health problem. Small ruminants are the preferred hosts of Brucella melitensis and thus the main source of human infections. Effective control of sheep and goat brucellosis has been achieved in several countries through vaccination with the live‐ attenuated B. melitensis Rev1 vaccine. However, Rev1 induces a long‐lasting serological response that hinders the differentiation between infected and vaccinated animals. A Rev1::gfp strain expressing constitutively the Green Fluorescent Protein (GFP) was built by stable insertion of a mini‐Tn7‐gfp in the glmS-recG non‐codifying chromosomal region. An associated indirect ELISA‐GFP was developed to identify anti‐GFP antibodies in vaccinated animals. The resulting Rev1::gfp kept the biological properties of the Rev1 reference strain, including residual virulence and efficacy in mice, and was readily distinguished from Rev1 and other Brucella field strains by direct visualization under ultraviolet illumination, fluorescence microscopy and a multiplex PCR‐GFP. The Rev1::gfp strain did not elicit anti‐GFP antibodies itself in lambs but when applied in combination with recombinant GFP induced an intense and long‐lasting (>9 months) anti‐GFP serological response readily detectable by the ELISA‐GFP. Overall, our results confirm that Rev1 GFP‐tagging can be a suitable alternative for identifying vaccinated sheep in infected contexts.es_ES
dc.description.sponsorshipSecretaría de Estado de Investigación, Desarrollo e Innovación, Grant/Award Number: AGL2011-30453-C04-01, AGL2014-58795-C04, RTC-2015-3618-1; CSIC-CRUSA Foundation bilateral project, Grant/Award Number: 2010CR0005es_ES
dc.language.isoenges_ES
dc.publisherWileyes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectBrucella melitensises_ES
dc.subjectELISA-GFPes_ES
dc.subjectMini-Tn7-gfpes_ES
dc.subjectSheepes_ES
dc.subjectVaccineses_ES
dc.titleGFP tagging of Brucella melitensis Rev1 allows the identification of vaccinated sheepes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.noteThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.es_ES
dc.identifier.doi10.1111/tbed.13053-
dadun.citation.endingPage516es_ES
dadun.citation.number66es_ES
dadun.citation.publicationNameTransboundary and Emerging Diseaseses_ES
dadun.citation.startingPage505es_ES
dadun.citation.volume2019es_ES

Files in This Item:
Thumbnail
File
Transbounding Emerging Dis - 2018 - Zabalza‐Barangu - GFP tagging of Brucella melitensis Rev1 allows the identification of.pdf
Description
Size
1.07 MB
Format
Adobe PDF


Items in Dadun are protected by copyright, with all rights reserved, unless otherwise indicated.