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dc.creatorAlonso-Jáuregui, M. (María)-
dc.creatorGonzalez-Peñas, E. (Elena)-
dc.creatorLopez-de-Cerain, A. (Adela)-
dc.creatorVettorazzi, A. (Ariane)-
dc.identifier.citationAlonso-Jáuregui, M. (María); González-Peñas, M. (María Elena); López de Cerain-Salsamendi, A. (Adela María); et al. "Genotoxicity of 12 mycotoxins by the SOS/umu test: comparison of liver and kidney S9 fraction". Toxins. 14 (6), 2022, 400es
dc.description.abstractLiver S9 fraction is usually employed in mutagenicity/genotoxicity in vitro assays, but some genotoxic compounds may need another type of bioactivation. In the present work, an alternative S9 fraction from the kidneys was used for the genotoxicity assessment of 12 mycotoxins with the SOS/umu test. The results were compared with liver S9 fraction, and 2-4 independent experiments were performed with each mycotoxin. The expected results were obtained with positive controls (4-nitroquinoline-N-oxide and 2-aminoanthracene) without metabolic activation or with liver S9, but a potent dose-dependent effect with 4-nitroquinoline-N-oxide and no activity of 2-aminoanthracene with kidney S9 were noticed. Aflatoxin B1 was genotoxic with metabolic activation, the effect being greater with liver S9. Sterigmatocystin was clearly genotoxic with liver S9 but equivocal with kidney S9. Ochratoxin A, zearalenone and fumonisin B1 were negative in all conditions. Trichothecenes were negative, except for nivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, T-2 and HT-2 toxins, which showed equivocal results with kidney S9 because a clear dose-response effect was not observed. Most of the mycotoxins have been assessed with kidney S9 and the SOS/umu test for the first time here. The results with the positive controls and the mycotoxins confirm that the organ used for the S9 fraction preparation has an influence on the genotoxic activity of some compounds.-
dc.description.sponsorshipThis work was funded by the Spanish “Ministerio de Economía, Industria y Competitividad, Agencia Estatal de Investigación” (AGL2017-85732-R) (MINECO/AEI/FEDER, UE). M.A.-J. thanks the “Asociación de Amigos” (University of Navarra) and the Spanish Government for the predoctoral grants (PRE2018-083527)”-
dc.subjectLiver S9-
dc.subjectKidney S9-
dc.subjectIn vitro-
dc.titleGenotoxicity of 12 mycotoxins by the SOS/umu test: comparison of liver and kidney S9 fraction-
dc.description.noteThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) licens-

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