A lncRNA-SWI/SNF complex crosstalk controls transcriptional activation at specific promoter regions
Keywords: 
lncRNA-SWI/SNF
SWI/SNF complexes
RNA interactome of SMARCB1
Issue Date: 
2020
Publisher: 
Springer Nature
Project: 
ERC-2017-COG 771425
info:eu-repo/grantAgreement/AEI/Proyectos I+D/BFU2017-82773-P/ES/EXPLORANDO LA FUNCION DEL TRANSCRIPTOMA LARGO NO CODIFICANTE EN LA REPLICACION DE LA CROMATINA HUMANA/
ISSN: 
2041-1723
32071317
Note: 
This article is licensed under a Creative Commons Attribution 4.0 International License
Citation: 
Grossi, E. (Elena); Raimondi, I. (Iván); Goñi, E. (Enrique); et al. "A lncRNA-SWI/SNF complex crosstalk controls transcriptional activation at specific promoter regions". Nature Communications. 11, 2020, 936
Abstract
LncRNAs have been shown to be direct players in chromatin regulation, but little is known about their role at active genomic loci. We investigate the role of lncRNAs in gene activation by profiling the RNA interactome of SMARCB1-containing SWI/SNF complexes in proliferating and senescent conditions. The isolation of SMARCB1-associated transcripts, together with chromatin profiling, shows prevalent association to active regions where SMARCB1 differentially binds locally transcribed RNAs. We identify SWINGN, a lncRNA interacting with SMARCB1 exclusively in proliferating conditions, exerting a pro-oncogenic role in some tumor types. SWINGN is transcribed from an enhancer and modulates the activation of GAS6 oncogene as part of a topologically organized region, as well as a larger network of pro-oncogenic genes by favoring SMARCB1 binding. Our results indicate that SWINGN influences the ability of the SWI/SNF complexes to drive epigenetic activation of specific promoters, suggesting a SWI/SNF-RNA cooperation to achieve optimal transcriptional activation.

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