Full metadata record
DC Field | Value | Language |
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dc.creator | Calvo, A. (Alba) | - |
dc.creator | Moreno, E. (Esther) | - |
dc.creator | Larrea, E. (Esther) | - |
dc.creator | Sanmartin-Grijalba, C. (Carmen) | - |
dc.creator | Irache, J.M. (Juan Manuel) | - |
dc.creator | Espuelas, S. (Socorro) | - |
dc.date.accessioned | 2023-02-28T07:46:15Z | - |
dc.date.available | 2023-02-28T07:46:15Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Calvo, A. (Alba); Moreno, E. (Esther); Larrea, E. (Esther); et al. "Berberine-Loaded Liposomes for the Treatment of Leishmania infantum-Infected BALB/c Mice". Pharmaceutics. 12 (9), 2020, | es_ES |
dc.identifier.issn | 1999-4923 | - |
dc.identifier.uri | https://hdl.handle.net/10171/65575 | - |
dc.description.abstract | Berberine (BER)—an anti-inflammatory quaternary isoquinoline alkaloid extracted from plants—has been reported to have a variety of biologic properties, including antileishmanial activity. This work addresses the preparation of BER-loaded liposomes with the aim to prevent its rapid liver metabolism and improve the drug selective delivery to the infected organs in visceral leishmaniasis (VL). BER liposomes (LP-BER) displayed a mean size of 120 nm, negative Z-potential of −38 mV and loaded 6 nmol/µmol lipid. In vitro, the loading of BER in liposomes enhanced its selectivity index more than 7-fold by decreasing its cytotoxicity to macrophages. In mice, LP-BER enhanced drug accumulation in the liver and the spleen. Consequently, the liposomal delivery of the drug reduced parasite burden in the liver and spleen by three and one logarithms (99.2 and 93.5%), whereas the free drug only decreased the infection in the liver by 1-log. The organ drug concentrations—far from IC50 values— indicate that BER immunomodulatory activity or drug metabolites also contribute to the efficacy. Although LP-BER decreased 10-fold—an extremely rapid clearance of the free drug in mice—the value remains very high. Moreover, LP-BER reduced plasma triglycerides levels. | es_ES |
dc.description.sponsorship | The work was supported by the Government of Navarra (Ayudas a Centros Tecnológicos y Organismos de Investigación y difusión de conocimientos para la realización de proyectos de I+D, PI045, PI068), the Institute of Tropical Health (ISTUN, University of Navarra) and funders (Obra Social La Caixa and Fundación Caja Navarra, Fundación Roviralta, PROFAND, Ubesol, ACUNSA and Artai). | es_ES |
dc.language.iso | eng | es_ES |
dc.relation | info:eu-repo/grantAgreement/Gobierno de Aragón/Subvenciones para el desarrollo de proyectos de investigación: Proyectos multidisciplinares/PI045%2F09/ES/Desarrollo de un sistema portatil Laser para la determinación de metales en minerales in situ en las minas de Tierga Zaragoza | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Berberine | es_ES |
dc.subject | Liposomes | es_ES |
dc.subject | Visceral leishmaniasis | es_ES |
dc.subject | Leishmania infantum | es_ES |
dc.title | Berberine-Loaded Liposomes for the Treatment of Leishmania infantum-Infected BALB/c Mice | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.publisher.place | Navarra | es_ES |
dc.description.note | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | es_ES |
dc.identifier.doi | 10.3390/pharmaceutics12090858 | - |
dadun.citation.number | 9 | es_ES |
dadun.citation.publicationName | Pharmaceutics | es_ES |
dadun.citation.volume | 12 | es_ES |
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