Automated database-guided expert-supervised orientation for immunophenotypic diagnosis and classification of acute leukemia

Lhermitte, L. (Ludovic); Mejstrikova, E. (Ester); Sluijs-Gelling, A. (Alita) van der; Grigore, G. (Georgiana); Sedek, L. (Lukasz); Bras, A.E. (Anne E.); Gaipa, G. (Giuseppe); Sobral-da-Costa, E. (Elaine); Novakova, M. (Michaela); Sonneveld, E. (E.); Buracchi, C. (Chiara); Sá-Bacelar, T. (T.) de; Marvelde, J. (Jeroen) te; Trinquand, A. (A.); Asnaf, V. (V.); Szczepanski, T. (Tomasz); Matarraz, S. (Sergio); Lopez, A. (A.); Vidriales, M.B. (María Belén); Bulsa, J. (J.); Hrusak, O. (O.); Kalina, T. (Tomas); Lecrevisse, Q. (Quentin); Martin-Ayuso, M. (M.); Brüggemann, M. (Monika); Verde, J. (Javier); Fernandez, P. (P.); Burgos, L. (Leire); Paiva, B. (Bruno); Pedreira, C.E. (Carlos E.); Dongen, J.J.M. (Jacques J. M.) van; Orfao, A. (Alberto); Velden, V.H.J. (Vicent H. J.) van der

Keywords:

Acute leukemia (AL)
AL orientation tube (ALOT)
B-cell precursor (BCP)
T-cell acute lymphoblastic leukemia (T-ALL)

Issue Date:

2018

Publisher:

Springer Nature

Project:

info:eu-repo/grantAgreement/MINECO/Ayudas a la incorporación de nuevas áreas temáticas y nuevos grupos al Consorcio CIBER (AE de Salud 2016)/CB16/12/00400/ES/CANCER

ISSN:

0887-6924

Note:

This work is licensed under a Creative Commons AttributionNonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http:// creativecommons.org/licenses/by-nc-nd/4.0/

Citation:

Lhermitte, L. (Ludovic); Mejstrikova, E. (Ester); Sluijs-Gelling, A. (Alita) van der; et al. "Automated database-guided expert-supervised orientation for immunophenotypic diagnosis and classification of acute leukemia". Leukemia. 32 (4), 2018, 874 - 881

Abstract

Precise classification of acute leukemia (AL) is crucial for adequate treatment. EuroFlow has previously designed an AL orientation tube (ALOT) to guide towards the relevant classification panel (T-cell acute lymphoblastic leukemia (T-ALL), B-cell precursor (BCP)-ALL and/or acute myeloid leukemia (AML)) and final diagnosis. Now we built a reference database with 656 typical AL samples (145 T-ALL, 377 BCP-ALL, 134 AML), processed and analyzed via standardized protocols. Using principal component analysis (PCA)-based plots and automated classification algorithms for direct comparison of single-cells from individual patients against the database, another 783 cases were subsequently evaluated. Depending on the database-guided results, patients were categorized as: (i) typical T, B or Myeloid without or; (ii) with a transitional component to another lineage; (iii) atypical; or (iv) mixed-lineage. Using this automated algorithm, in 781/783 cases (99.7%) the right panel was selected, and data comparable to the final WHO-diagnosis was already provided in >93% of cases (85% T-ALL, 97% BCP-ALL, 95% AML and 87% mixed-phenotype AL patients), even without data on the full-characterization panels. Our results show that database-guided analysis facilitates standardized interpretation of ALOT results and allows accurate selection of the relevant classification panels, hence providing a solid basis for designing future WHO AL classifications.

URI:

https://hdl.handle.net/10171/65650

DOI:

10.1038/leu.2017.313

Appears in Collections:

DA - CIMA - Inmunología e inmunoterapia - Inmunología del cáncer - Artículos de revista

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