MRGPRX2-mediated mast cell response to drugs used in perioperative procedures and anaesthesia

info:eu-repo/grantAgreement/MINECO/Proyectos de I+D+I dentro del Programa Estatal Retos de la Sociedad (2015)/SAF2015-68124-R/ES/REGULACION DE LA EXPRESION Y FUNCION DE KIT Y PDGFRALFA POR LA MOLECULA ADAPTADORA 3BP2. IMPLICACION EN LA DIFERENCIACION Y MIGRACION MASTOCITARIA Y EN PATOLOGIA

ISSN:

2045-2322

Note:

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Citation:

Navinés-Ferrer, A. (Arnau); Serrano-Candelas, E. (Eva); Lafuente, A. (A.); et al. "MRGPRX2-mediated mast cell response to drugs used in perioperative procedures and anaesthesia". Scientific reports. 8 (1), 2018, 11628

Abstract

The study of anaphylactoid reactions during perioperative procedures and anaesthesia represents a diagnostic challenge for allergists, as many drugs are administered simultaneously, and approximately half of them trigger allergic reactions without a verifiable IgE-mediated mechanism. Recently, mast cell receptor MRGPRX2 has been identified as a cause of pseudo-allergic drug reactions. In this study, we analyse the ability of certain drugs used during perioperative procedures and anaesthesia to induce MRGPRX2-dependent degranulation in human mast cells and sera from patients who experienced an anaphylactoid reaction during the perioperative procedure. Using a β-hexosaminidase release assay, several drugs were seen to cause mast cell degranulation in vitro in comparison with unstimulated cells, but only morphine, vancomycin and cisatracurium specifically triggered this receptor, as assessed by the release of β-hexosaminidase in the control versus the MRGPRX2-silenced cells. The same outcome was seen when measuring degranulation based on the percentage of CD63 expression at identical doses. Unlike that of the healthy controls, the sera of patients who had experienced an anaphylactoid reaction induced mast-cell degranulation. The degranulation ability of these sera decreased when MRGPRX2 was silenced. In conclusion, MRGPRX2 is a candidate for consideration in non-IgE-mediated allergic reactions to some perioperative drugs, reinforcing its role in mast cell responses and their pathophysiology.

URI:

https://hdl.handle.net/10171/65666

DOI:

10.1038/s41598-018-29965-8

Appears in Collections:

DA - CUN - Anestesia y cuidados intensivos - Artículos de revista

Files in This Item: