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dc.creatorIbrahim, A. (Amel)-
dc.creatorRodriguez-Florez, N. (Naiara)-
dc.creatorGardner, O.F.W. (Oliver F.W.)-
dc.creatorZucchelli, E. (Eleonora)-
dc.creatorNew, S.E.P. (Sophie E. P.)-
dc.creatorBorghi, A. (Alessandro)-
dc.creatorDunaway, D.J. (David J.)-
dc.creatorBulstrode, N.W. (Neil W.)-
dc.creatorFerretti, P. (Patrizia)-
dc.identifier.citationIbrahim, A. (Amel); Rodriguez-Florez, N. (Naiara); Gardner, O.F.W. (Oliver F.W.); et al. "Three-dimensionalenvironment and vascularization induce osteogenic maturation of human adipose-derived stem cells comparable to that of bone-derived progenitors". Stem cells translational medicine. 9 (12), 2020, 1651 - 1666es
dc.description.abstractWhile human adipose-derived stem cells (hADSCs) are known to possess osteogenic differentiation potential, the bone tissues formed are generally considered rudimentary and immature compared with those made by bone-derived precursor cells such as human bone marrow-derived mesenchymal stem cells (hBMSCs) and less commonly studied human calvarium osteoprogenitor cells (hOPs). Traditional differentiation protocols have tended to focus on osteoinduction of hADSCs through the addition of osteogenic differentiation media or use of stimulatory bioactive scaffolds which have not resulted in mature bone formation. Here, we tested the hypothesis that by reproducing the physical as well as biochemical bone microenvironment through the use of three-dimensional (3D) culture and vascularization we could enhance osteogenic maturation in hADSCs. In addition to biomolecular characterization, we performed structural analysis through extracellular collagen alignment and mineral density in our bone tissue engineered samples to evaluate osteogenic maturation. We further compared bone formed by hADSCs, hBMSCs, and hOPs against mature human pediatric calvarial bone, yet not extensively investigated. Although bone generated by all three cell types was still less mature than native pediatric bone, a fibrin-based 3D microenvironment together with vascularization boosted osteogenic maturation of hADSC making it similar to that of bone-derived osteoprogenitors. This demonstrates the important role of vascularization and 3D culture in driving osteogenic maturation of cells easily available but constitutively less committed to this lineage and suggests a crucial avenue for recreating the bone microenvironment for tissue engineering of mature craniofacial bone tissues from pediatric hADSCs, as well as hBMSCs and hOPs.es_ES
dc.description.sponsorshipThis work was supported by the RCS Blond Research Training Fellowship, RCS Fulbright Scholarship, Newlife Foundation, Great Ormond Street Hospital for Children Charity, UCL Impact Studentship, GOSH NIHR Biomedical Research Centre, and European Research Council. We are most grateful to Dr Dale Moulding at the ICH Microscopy Facility for his advice on acquisition and analysis of the results. We also wish to thank to our colleagues Leonardo Guasti, N.U. Owase Jeelani, Silvia Schievano, Deborah Eastwood, Timothy Arnett, and Michelangelo Corcelli for helpful discussions, mentorship, and providing access and advice on μCT. This report incorporates independent research from the National Institute for Health Research Biomedical Research Centre Funding Scheme. The views expressed in this publication are those of the author(s) and not necessarily those of the NHS, the National Institute for Health Research or the Department of Health.es_ES
dc.subject3D environmentes_ES
dc.subjectHuman adipose-derived stem ces_ES
dc.titleThree-dimensionalenvironment and vascularization induce osteogenic maturation of human adipose-derived stem cells comparable to that of bone-derived progenitorses_ES
dc.description.noteThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.es_ES
dadun.citation.publicationNameStem cells translational medicinees_ES

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