Full metadata record
DC FieldValueLanguage
dc.creatorArredondo-Montero, J. (Javier)-
dc.creatorTorres-López, A. (Andrea)-
dc.creatorHurtado-Ilzarbe, G. (Guillermina)-
dc.creatorAntona, G. (Giuseppa)-
dc.creatorRos-Briones, R. (Raquel)-
dc.creatorLopez-Andres, N. (Natalia)-
dc.creatorMartin-Calvo, N. (Nerea)-
dc.date.accessioned2023-11-15T16:18:06Z-
dc.date.available2023-11-15T16:18:06Z-
dc.date.issued2023-
dc.identifier.citationArredondo Montero, J.; López, A. T.; Ilzarbe, G. H.; et al. "Flow cytometric characterization of cecal appendix lymphocyte subpopulations in children: a pilot study". Pediatric Surgery International. 39 (1), 2023, 274es
dc.identifier.issn0179-0358-
dc.identifier.urihttps://hdl.handle.net/10171/67859-
dc.description.abstractIntroductionScientific literature regarding the characterization of lymphocyte subpopulations of the cecal appendix is sparse, with few precedents limited to immunohistochemical techniques.MethodsWe conducted a prospective pilot study to characterize lymphocyte subpopulations of the cecal appendix in children. Participants were divided into three groups: (1) patients without histological acute appendiceal inflammation, (2) patients with histological uncomplicated acute appendicitis, and (3) patients with histological complicated acute appendicitis (gangrenous, perforated). A fresh sample of the base of the appendix was taken from all patients and a flow cytometric study was performed. Quantitative variables were compared using Kruskal-Wallis test and Mann-Whitney U test.ResultsThis study included 57 patients divided into Group 1 (n = 5), Group 2 (n = 37), and Group 3 (n = 15). Median values (IQR) of the percentage of B-lymphocytes were 67.8 [66.8-68.1] in group 1, 61.15 [53.74-66.4] in group 2, and 52.1 [33-62.02] in group 3 (p = 0.02). Median values (IQR) of the percentage of NK-lymphocytes were 0.26 [0.2-0.3] in group 1, 0.55 [0.37-0.66] in group 2, and 0.84 [0.35-1.45] in group 3 (p = 0.008). Median values (IQR) of the percentage of T-lymphocytes were 31.9 [31.7-33.1] in group 1, 37.68 [32.15-45.69] in group 2, and 46.9 [37.03-67] in group 3 (p = 0.02). Pair comparisons of groups 2 and 3 also showed significant differences in the percentage of B lymphocytes (p = 0.03) and NK-lymphocytes (p = 0.02).ConclusionsSignificant differences in lymphocyte subpopulations were identified according to the histologic grade of the cecal appendix. More specifically, a lower percentage of B-lymphocytes and a higher percentage of T- and NK-lymphocytes were observed in cases of acute appendicitis. These findings must be confirmed and their etiopathogenic, diagnostic, and prognostic implications elucidated in future studies with larger sample sizes.-
dc.language.isoen-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.subjectB-lymphocytes-
dc.subjectFlow cytometry-
dc.subjectNK-lymphocytes-
dc.subjectPediatric acute appendicitis-
dc.subjectT-lymphocytes-
dc.titleFlow cytometric characterization of cecal appendix lymphocyte subpopulations in children: a pilot study-
dc.typeinfo:eu-repo/semantics/article-
dc.description.noteThis article is licensed under a Creative Commons Attribution 4.0 International License-
dc.identifier.doi10.1007/s00383-023-05558-z-
dadun.citation.number1-
dadun.citation.publicationNamePediatric Surgery International-
dadun.citation.startingPage274-
dadun.citation.volume39-
dc.identifier.pmid37736768-

Files in This Item:
Thumbnail
File
pdf.pdf
Description
Size
2.64 MB
Format
Adobe PDF


Statistics and impact
0 citas en
0 citas en

Items in Dadun are protected by copyright, with all rights reserved, unless otherwise indicated.