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dc.creatorGil-Korilis, A. (Adrián)-
dc.creatorCojocaru, M. (Mihail)-
dc.creatorBerzosa-Suñer, M. (Melibea)-
dc.creatorGamazo, C. (Carlos)-
dc.creatorAndrade, N.J. (Natália J.)-
dc.creatorCiuffi, K.J. (Katia J.)-
dc.date.accessioned2023-11-21T10:12:22Z-
dc.date.available2023-11-21T10:12:22Z-
dc.date.issued2023-
dc.identifier.citationGil-Korilis, A. (Adrián); Cojocaru, M. (Mihail); Berzosa-Suñer, M. (Melibea); et al. "Comparison of antibacterial activity and cytotoxicity of silver nanoparticles and silver-loaded montmorillonite and saponite". Applied Clay Science. 240, 2023, 106968es
dc.identifier.issn1872-9053-
dc.identifier.urihttps://hdl.handle.net/10171/67908-
dc.description.abstractAlthough silver nanoparticles are known for their antibacterial activity, little research has been carried out on what synthesis method provides the most effective particles. In this study, silver nanoparticles were synthesised via chemical reduction by using silver nitrate as the silver precursor, ascorbic acid as the reducing agent and sodium citrate as the stabilising agent. The solutions were adjusted to several pH values employing sodium hydroxide, citric acid or nitric acid. Dynamic light scattering and absorption spectra in the ultraviolet/visible region characterisation revealed that employing nitric acid to adjust the pH produced more varied and larger silver particle sizes. Then, silver nanoparticles were supported on montmorillonite and saponite through wet impregnation or ion exchange methods. Scanning electron microscopy, energy-dispersive X-ray spectroscopy and transmission electron microscopy characterisation confirmed that silver nanoparticles were successfully loaded onto the clay minerals. Next, the antibacterial activity of the samples was evaluated against Escherichia coli and Staphylococcus aureus by determining their minimum inhibitory concentrations and minimum bactericidal concentrations. The free silver nanoparticles did not show any antibacterial activity at 125 mg/L. In contrast, the silver-loaded samples obtained by wet impregnation and with a higher silver content displayed the strongest antibacterial effect. Finally, the cytotoxicity of the samples was determined in GM07492-A cell line by using an XTT colorimetric assay. The calculated IC50 values revealed that the supported silver nanoparticles were barely toxic. Thus, the silver-loaded clay minerals obtained here are promising antibacterial materials with a high-grade safety profile.es_ES
dc.description.sponsorshipNJA and KJC acknowledge the support from research funding agencies Fundaçao de Amparo a Pesquisa do Estado de Sao Paulo, FAPESP (2020/06712-6), Coordenaçao de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Finance Code 001 and Conselho Nacional de Desenvolvimento Científico e Tecnologico, CNPq (305180/2019-3)es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relation2020/06712-6es_ES
dc.relation305180/2019-3es_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectSilver nanoparticleses_ES
dc.subjectClay mineralses_ES
dc.subjectMontmorillonitees_ES
dc.subjectSaponitees_ES
dc.subjectAntibacterial activityes_ES
dc.subjectCytotoxicityes_ES
dc.titleComparison of antibacterial activity and cytotoxicity of silver nanoparticles and silver-loaded montmorillonite and saponitees_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.noteThis is an open access article under the CC BY-NC-ND licensees_ES
dc.identifier.doi10.1016/j.clay.2023.106968-
dadun.citation.publicationNameApplied Clay Sciencees_ES
dadun.citation.startingPage106968es_ES
dadun.citation.volume240es_ES

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