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dc.creatorArredondo-Montero, J. (Javier)-
dc.creatorLópez, A. (Andrés)-
dc.creatorHurtado-Ilzarbe, G. (Guillermina)-
dc.creatorAntona, G. (Giuseppa)-
dc.creatorRos-Briones, R. (Raquel)-
dc.creatorLopez-Andres, N. (Natalia)-
dc.creatorMartin-Calvo, N. (Nerea)-
dc.date.accessioned2023-12-22T09:33:04Z-
dc.date.available2023-12-22T09:33:04Z-
dc.date.issued2023-
dc.identifier.citationArredondo-Montero, J. (Javier); Torres-López, A. (Andrea); Hurtado-Ilzarbe, G. (Guillermina); et al. "Flow cytometric characterization of cecal appendix lymphocyte subpopulations in children: a pilot study". Pediatric Surgery International. 39 (274), 2023,es_ES
dc.identifier.issn1437-9813-
dc.identifier.urihttps://hdl.handle.net/10171/68172-
dc.description.abstractIntroduction: Scientific literature regarding the characterization of lymphocyte subpopulations of the cecal appendix is sparse, with few precedents limited to immunohistochemical techniques. Methods: We conducted a prospective pilot study to characterize lymphocyte subpopulations of the cecal appendix in children. Participants were divided into three groups: (1) patients without histological acute appendiceal inflammation, (2) patients with histological uncomplicated acute appendicitis, and (3) patients with histological complicated acute appendicitis (gangrenous, perforated). A fresh sample of the base of the appendix was taken from all patients and a flow cytometric study was performed. Quantitative variables were compared using Kruskal-Wallis test and Mann-Whitney U test. Results: This study included 57 patients divided into Group 1 (n = 5), Group 2 (n = 37), and Group 3 (n = 15). Median values (IQR) of the percentage of B-lymphocytes were 67.8 [66.8-68.1] in group 1, 61.15 [53.74-66.4] in group 2, and 52.1 [33-62.02] in group 3 (p = 0.02). Median values (IQR) of the percentage of NK-lymphocytes were 0.26 [0.2-0.3] in group 1, 0.55 [0.37-0.66] in group 2, and 0.84 [0.35-1.45] in group 3 (p = 0.008). Median values (IQR) of the percentage of T-lymphocytes were 31.9 [31.7-33.1] in group 1, 37.68 [32.15-45.69] in group 2, and 46.9 [37.03-67] in group 3 (p = 0.02). Pair comparisons of groups 2 and 3 also showed significant differences in the percentage of B lymphocytes (p = 0.03) and NK-lymphocytes (p = 0.02). Conclusions: Significant differences in lymphocyte subpopulations were identified according to the histologic grade of the cecal appendix. More specifically, a lower percentage of B-lymphocytes and a higher percentage of T- and NK-lymphocytes were observed in cases of acute appendicitis. These findings must be confirmed and their etiopathogenic, diagnostic, and prognostic implications elucidated in future studies with larger sample sizes.es_ES
dc.description.sponsorshipOpen Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature.es_ES
dc.language.isoenges_ES
dc.publisherSpringeres_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectB-lymphocyteses_ES
dc.subjectFlow cytometryes_ES
dc.subjectNK-lymphocyteses_ES
dc.subjectPediatric acute appendicitises_ES
dc.subjectT-lymphocyteses_ES
dc.titleFlow cytometric characterization of cecal appendix lymphocyte subpopulations in children: a pilot studyes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.noteThis article is licensed under a Creative Commons Attribution 4.0 International Licensees_ES
dc.identifier.doi10.1007/s00383-023-05558-z-
dadun.citation.number274es_ES
dadun.citation.publicationNamePediatric Surgery Internationales_ES
dadun.citation.volume39es_ES
dc.identifier.pmid37736768-

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