Study of circulating MicroRNA-125b levels in serum exosomes in advanced melanoma
Keywords: 
Malignant melanoma
Exosomes
MicroRNAs (miRNAs)
Tumoral cell dysregulation
Issue Date: 
2014
Publisher: 
College of american pathologists
Project: 
info:eu-repo/grantAgreement/MICINN/Proyectos de Investigación en Salud/PI11%2F02119/ES/Búsqueda de marcadores tumorales asociados a exosomas circulantes en pacientes con melanoma
ISSN: 
0003-9985
Citation: 
Alegre, E. (Estibaliz); Fernandez-Sanmamed, M. (Miguel); Rodríguez, C. (Carmen); et al. "Study of circulating MicroRNA-125b levels in serum exosomes in advanced melanoma". Archives of Pathology & Laboratory Medicine. 138 (6), 2014, 828 - 832
Abstract
Context: Malignant melanoma is an aggressive tumor that produces exosomes, which contain microRNAs (miRNAs) that could be of utility in following tumoral cell dysregulation. MicroR-125b is a miRNA whose down-regulation seems to be implicated in melanoma progression. Objective: To analyze miR-125b levels in serum, and in exosomes obtained from serum, from patients with advanced melanoma. Design: Serum samples were obtained from 21 patients with advanced melanoma, from 16 disease-free patients with melanoma, and from 19 healthy volunteers. Exosomes were isolated from serum by precipitation, and miR-16 and miR-125b levels were quantified by real-time polymerase chain reaction. Results: MicroR-16, but not miR-125b, was detected in all samples, and miR-16 levels were significantly higher in serum than they were in exosomes. MicroR-16 expression levels did not differ significantly between the 2 groups (patients with melanoma and healthy donors). There was a significant relationship between miR-125b and miR-16 levels in exosomes. Additionally, miR-125b levels in exosomes were significantly lower in patients with melanoma compared with disease-free patients with melanoma and healthy controls. Conclusions: Exosomes can provide a suitable material to measure circulating miRNA in melanoma, and miR-16 can be used as an endogenous normalizer. Lower levels of miR-125b in exosomes obtained from serum are associated with advanced melanoma disease, probably reflecting the tumoral cell dysregulation.

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