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dc.creatorSabaté-Brescó, M. (Marina)-
dc.creatorQuan, P.L. (Paola Leonor)-
dc.creatorD'Amelio-Garofalo, C.M. (Carmen Mariana)-
dc.creatorPascal, M. (Mariona)-
dc.creatorGarcia, B.E. (Blanca Esther)-
dc.creatorGastaminza, G. (Gabriel)-
dc.creatorBlanca-López, N. (Natalia)-
dc.creatorAlvarado, M.I. (María Isabel)-
dc.creatorFernandez, J. (Javier)-
dc.creatorMoya, C. (Carmen)-
dc.creatorBartra, J. (Joan)-
dc.creatorFerrer-Cardona, M. (Marta)-
dc.creatorGoikoetxea-Lapresa, M.J. (María José)-
dc.date.accessioned2024-02-02T13:40:57Z-
dc.date.available2024-02-02T13:40:57Z-
dc.date.issued2022-
dc.identifier.citationSabaté-Brescó, M. (Marina); Quan, P.L. (Paola Leonor); D'Amelio-Garofalo, C.M. (Carmen Mariana); et al. "Validation of a commercial allergen microarray platform for specific immunoglobulin E detection of respiratory and plant food allergens". Annals of Allergy, Asthma & Immunology. 128 (3), 2022, 283 - 290es_ES
dc.identifier.issn1081-1206-
dc.identifier.urihttps://hdl.handle.net/10171/68733-
dc.description.abstractBackground As the use of multiplex-specific immunoglobulin E (sIgE) detection methods becomes increasingly widespread, proper comparative validation assessments of emerging new platforms are vital. Objective To evaluate the clinical and technical performance of a newly introduced microarray platform, Allergy Explorer (ALEX) (MacroArray Diagnostics), in the diagnosis of pollen (cypress, grass, olive), dust mite (Dermatophagoides pteronyssinus), mold (Alternaria alternata), fruit (apple, peach), and nut (walnut, hazelnut and peanut) allergies and to compare it with those of the ImmunoCAP Immuno Solid-phase Allergen Chip (ISAC) 112 microarray and the ImmunoCAP singleplex method (ThermoFisher Scientific). Methods We enrolled 153 patients with allergy and 16 controls without atopy. The sIgE assays were conducted using ISAC112, ALEX version 2 (ALEX2), and ImmunoCAP for whole extracts and major components. Technical validation of ALEX2 was performed by measuring repeatability and interassay, interbatch, and interlaboratory reproducibility. Results When measured globally (detection by 1 or more allergen components), ALEX2 had adequate sensitivity and specificity for most of the allergens studied, comparable in general with that of ISAC112 (except for olive pollen and walnut) and similar to that of ImmunoCAP whole extract measurements. Component-by-component analysis revealed comparable results for all techniques, except for Ole e 1 and Jug r 3, in both ISAC112 and ImmunoCAP comparisons, and Alt a 1, when compared with ISAC112. Continuous sIgE levels correlate with sIgE by ImmunoCAP. Good reproducibility and repeatability were observed for ALEX2. Conclusion ALEX2 has sound technical performance and adequate diagnostic capacity, comparable in general with that of ISAC112 and ImmunoCAP.es_ES
dc.description.sponsorshipThis research was financially supported by the Carlos III Health Institute (grant: PI 11/01634), the Sociedad Espanola de Alergia e Inmunologia Clínica Foundation, and the ~ Carlos III Health Institute through the Spanish Research Network on Adverse Reactions to Allergens and Drugs (RIRAAF, RD12/0013/00010, and ARADYAL, RD16/0006/0031). MacroArray Diagnostics supplied all the ALEX2-related material required for this study and ThermoFisher Scientific provided most of the ImmunoCAP-related material. Dr Quan and Dr Sabate-Bresc o contributed equally to this work.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectAllergy Exploreres_ES
dc.subjectMacroArray Diagnosticses_ES
dc.subjectDiagnosis of pollenes_ES
dc.subjectDust mitees_ES
dc.subjectMoldes_ES
dc.subjectDermatophagoides pteronyssinuses_ES
dc.subjectAlternaria alternataes_ES
dc.subjectImmunoCAP Immuno Solid-phase Allergen Chip (ISAC)es_ES
dc.subjectThermoFisher Scientifices_ES
dc.subjectImmuno Solid-phase Allergen Chip (ISAC)es_ES
dc.titleValidation of a commercial allergen microarray platform for specific immunoglobulin E detection of respiratory and plant food allergenses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/abs/pii/S1081120621012886es_ES
dc.editorial.note© 2021 American College of Allergy, Asthma & Immunologyes_ES
dadun.citation.endingPage290es_ES
dadun.citation.number3es_ES
dadun.citation.publicationNameAnnals of Allergy, Asthma & Immunologyes_ES
dadun.citation.startingPage283es_ES
dadun.citation.volume128es_ES

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