Mutagenicity and genotoxicity assessment of the emerging mycotoxin Versicolorin A, an Aflatoxin B1 precursor
Keywords: 
Mycotoxins
Versicolorin A
Aflatoxin B1
Genotoxicity
Mutagenicity
DNA damage
Issue Date: 
2023
Publisher: 
Elsevier
ISSN: 
0269-7491
Note: 
CC BY-NC-ND
Citation: 
Al-Ayoubi, C. (Carine); Alonso-Jáuregui, M. (María); Azqueta, A. (Amaya); et al. "Mutagenicity and genotoxicity assessment of the emerging mycotoxin Versicolorin A, an Aflatoxin B1 precursor". Environmental Pollution. , 2023, 122276
Abstract
Aflatoxin B1 (AFB1) is the most potent natural carcinogen among mycotoxins. Versicolorin A (VerA) is a pre- cursor of AFB1 biosynthesis and is structurally related to the latter. Although VerA has already been identified as a genotoxin, data on the toxicity of VerA are still scarce, especially at low concentrations. The SOS/umu and miniaturised version of the Ames test in Salmonella Typhimurium strains used in the present study shows that VerA induces point mutations. This effect, like AFB1, depends primarily on metabolic activation of VerA. VerA also induced chromosomal damage in metabolically competent intestinal cells (IPEC-1) detected by the micro- nucleus assay. Furthermore, results from the standard and enzyme-modified comet assay confirmed the VerA- mediated DNA damage, and we observed that DNA repair pathways were activated upon exposure to VerA, as indicated by the phosphorylation and/or relocation of relevant DNA-repair biomarkers (γH2AX and 53BP1/ FANCD2, respectively). In conclusion, VerA induces DNA damage without affecting cell viability at concentra- tions as low as 0.03 μM, highlighting the danger associated with VerA exposure and calling for more research on the carcinogenicity of this emerging food contaminant.

Files in This Item:
File
revised version-Manuscript.pdf
Description
Size
1.26 MB
Format
Adobe PDF
The file will be visible since 24-Jul-2024


Statistics and impact

Items in Dadun are protected by copyright, with all rights reserved, unless otherwise indicated.