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dc.creatorSelva, L. (Laura)-
dc.creatorBenmessaoud, R. (Rachid)-
dc.creatorLanaspa, M. (Miguel)-
dc.creatorJroundi, I. (Imane)-
dc.creatorMoraleda, C. (Cinta)-
dc.creatorAcacio, S. (Sozinho)-
dc.creatorIñigo, M. (Melania)-
dc.creatorBastiani, A. (Alien)-
dc.creatorMonsonis, M. (Manuel)-
dc.creatorPallares, R. (Roman)-
dc.creatorBassat, Q. (Quique)-
dc.creatorMuñoz-Almagro, C. (Carmen)-
dc.identifier.citationSelva, L. (Laura); Benmessaoud, R. (Rachid); Lanaspa, M. (Miguel); et al. "Detection of Streptococcus pneumoniae and Haemophilus influenzae Type B by Real-Time PCR from Dried Blood Spot Samples among Children with Pneumonia: A Useful Approach for Developing Countries". PLoS ONE. 8 (10), 2013, e76970es
dc.description.abstractBackground: Dried blood spot (DBS) is a reliable blood collection method for storing samples at room temperature and easily transporting them. We have previously validated a Real-Time PCR for detection of Streptococcus pneumoniae in DBS. The objective of this study was to apply this methodology for the diagnosis of S. pneumoniae and Haemophilus influenzae b (Hib) in DBS samples of children with pneumonia admitted to two hospitals in Mozambique and Morocco. Methods: Ply and wzg genes of S. pneumoniae and bexA gene of Hib, were used as targets of Real-Time PCR. 329 DBS samples of children hospitalized with clinical diagnosis of pneumonia were tested. Results: Real-Time PCR in DBS allowed for a significant increase in microbiological diagnosis of S. pneumoniae and Hib. When performing blood bacterial culture, only ten isolates of S. pneumoniae and none of Hib were detected (3·0% positivity rate, IC95% 1·4-5·5%). Real-Time PCR from DBS samples increased the detection yield by 4x fold, as 30 S. pneumoniae and 11 Hib cases were detected (12·4% positivity rate, IC95% 9·0-16·5%; P<0·001). Conclusion: Real-Time PCR applied in DBS may be a valuable tool for improving diagnosis and surveillance of pneumonia caused by S. pneumoniae or Hib in developing countries.es_ES
dc.description.sponsorshipThis work was supported in part by Fondo de Investigaciones Sanitarias [PI10/02058 and a Research Grant to QB], Agència de Gestió d'Ajuts Universitaris i de Recerca [SGR00136 and FI-ICIP 2012 to LS] and Fundació Sant Joan de Déu [Research Grant to LS]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.es_ES
dc.publisherPublic Library of Science (PLoS)es_ES
dc.subjectMaterias Investigacion::Ciencias de la Saludes_ES
dc.subjectStreptococcus pneumoniaees_ES
dc.titleDetection of Streptococcus pneumoniae and Haemophilus influenzae Type B by Real-Time PCR from Dried Blood Spot Samples among Children with Pneumonia: A Useful Approach for Developing Countrieses_ES
dc.description.noteThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.es_ES
dadun.citation.publicationNamePLoS ONEes_ES

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