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dc.creatorChacón, M. (Manuel)-
dc.creatorVázquez, N. (Natalia)-
dc.creatorBerisa, S. (Silvia)-
dc.creatorPersinal, M. (Mairobi)-
dc.creatorSanchez, M. (Manuel)-
dc.creatorBaamonde, B. (Begoña)-
dc.creatorAlfonso, J.F. (José F.)-
dc.creatorFernández-Vega-Cueto, L. (Luis)-
dc.creatorMerayo-Lloves, J. (Jesús)-
dc.creatorMeana, A. (Álvaro)-
dc.date.accessioned2024-02-12T09:54:00Z-
dc.date.available2024-02-12T09:54:00Z-
dc.date.issued2019-
dc.identifier.citationChacón, M. (Manuel); Vázquez, N. (Natalia); Berisa, S. (Silvia); et al. "QobuR – A new in vitro human corneal epithelial model for preclinical drug screening". European Journal of Pharmaceutics and Biopharmaceutics. 136, 2019, 164 - 173es
dc.identifier.issn0939-6411-
dc.identifier.urihttps://hdl.handle.net/10171/69070-
dc.description.abstractA new in vitro human corneal epithelial model (QobuR) obtained from normal limbal tissue has been developed to study ocular irritancy of different ophthalmic compounded drugs. Phenotypical characterization and transepithelial electrical resistance (TEER) of QobuR revealed essential similarities compared with a native human cornea, displaying functional markers and TEER values near 1500 Ωcm2 at day 7th of cellular differentiation. Using this model, ocular irritancy and barrier integrity alterations were evaluated using MTT reaction and variations in TEER. We found that some of the Non-Irritant products evaluated still damage the corneal epithelial integrity and current protocols for ocular irritancy should therefore include a barrier integrity evaluation. Moreover, in order to comprehensively evaluate corneal permeability of the active ingredients, we propose the use of QobuR as an all-in-one alternative method for evaluating ocular irritancy, barrier disruptions and permeability rates of topically applied ocular drugs to improve current in vitro drug testing procedures.es_ES
dc.description.sponsorshipThis work was supported in part by grant FC-15-GRUPIN14-141 (Government of Principado de Asturias co financed by European Regional Development Fund (FEDER)) and grant: INNOVA-IDEPA-IDE2016-000215 (Government of Principado de Asturias). Authors wish to thank Centro Comunitario de Sangre y Tejidos de Asturias and DropSens S.L. for their assistance during the experiments.es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightsinfo:eu-repo/semantics/closedAccesses_ES
dc.subjectOcular irritancyes_ES
dc.subjectDrug absorptiones_ES
dc.subjectIn vitro cornea modeles_ES
dc.subjectQobuRes_ES
dc.subjectTEER measurementes_ES
dc.subjectBarrier disruptionses_ES
dc.subjectAlternative methodes_ES
dc.titleQobuR – A new in vitro human corneal epithelial model for preclinical drug screeninges_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.identifier.doi10.1016/j.ejpb.2019.01.023-
dadun.citation.endingPage173es_ES
dadun.citation.publicationNameEuropean Journal of Pharmaceutics and Biopharmaceuticses_ES
dadun.citation.startingPage164es_ES
dadun.citation.volume136es_ES

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