Full metadata record
DC FieldValueLanguage
dc.creatorRiestra, A.C. (A.C.)-
dc.creatorVázquez, N. (Natalia)-
dc.creatorChacón, M. (Manuel)-
dc.creatorBerisa, S. (Silvia)-
dc.creatorSánchez-Ávila, R.M. (Ronald M.)-
dc.creatorOrive, G. (Gorka)-
dc.creatorAnitua, E. (Eduardo)-
dc.creatorMeana, A. (Álvaro)-
dc.creatorMerayo-Lloves, J. (Jesús)-
dc.identifier.citationRiestra, A.C. (A.C.); Vázquez, N. (Natalia); Chacón, M. (Manuel); et al. "Autologous method for ex vivo expansion of human limbal epithelial progenitor cells based on plasma rich in growth factors technology". The Ocular Surface. 15 (2), 2017, 248 - 256es
dc.description.abstractPurpose Develop an autologous culture method for ex vivo expansion of human limbal epithelial progenitor cells (LEPCs) using Plasma Rich in Growth Factors (PRGF) as a growth supplement and as a scaffold for the culture of LEPCs. Methods LEPCs were cultivated in different media supplemented with 10% fetal bovine serum (FBS) or 10% PRGF. The outgrowths, total number of cells, colony forming efficiency (CFE), morphology and immunocytochemistry against p63- α and cytokeratins 3 and 12 (CK3-CK12) were analyzed. PRGF was also used to elaborate a fibrin membrane. The effects of the scaffold on the preservation of stemness and the phenotypic characterization of LEPCs were investigated through analysis of CK3-CK12, ABCG-2 and p63. Results LEPCs cultivated with PRGF showed a significantly higher growth area than FBS cultures. Moreover, the number of cells were also higher in PRGF than FBS, while displaying a better morphology overall. CFE was found to be also higher in PRGF groups compared to FBS, and the p63-α expression also differed between groups. LEPCs cultivated on PRGF membranes appeared as a confluent monolayer of cells and still retained p63 and ABCG-2 expression, being negative for CK3-CK12. Conclusions PRGF can be used in corneal tissue engineering, supplementing the culture media, even in a basal media without any other additives, as well as providing a scaffold for the culture.es_ES
dc.description.sponsorshipThis study was supported in part by Fundacion Telefonica, Spain and by Government of Spain co funded by European Social Fund, European Union (Ministerio de Ciencia e Innovacion Retos Colaboracion 2014, RTC-2014-2375-1). EA is the scientific director and GO is a researcher of the biotechnological company BTI.es_ES
dc.subjectCell culture techniqueses_ES
dc.subjectCell proliferationes_ES
dc.subjectCell transplantationes_ES
dc.subjectCorneal epitheliumes_ES
dc.subjectCulture mediaes_ES
dc.subjectLimbal deficiencyes_ES
dc.subjectLimbus corneaees_ES
dc.subjectPlasma rich in growth factorses_ES
dc.titleAutologous method for ex vivo expansion of human limbal epithelial progenitor cells based on plasma rich in growth factors technologyes_ES
dadun.citation.publicationNameThe Ocular Surfacees_ES

Files in This Item:
2017 Autologous method.pdf
2.56 MB
Adobe PDF

Statistics and impact
0 citas en
0 citas en

Items in Dadun are protected by copyright, with all rights reserved, unless otherwise indicated.