Bishop, M.R. (Michael R.)Iversen, P. L. (Patrick L.)Graham-Sharp, J. (J.)Martin-Algarra, S. (Salvador)Kessinger, A. (Anne)Verbik, D.J. (David J.)Jackson, J.D. (John D.)Wu, AI.G. (AI G.)Joshi, S.S. (Shantaram S.)Pirruccello, S.J. (Samuel J.)2024-02-082024-02-081996Joshi, S.S. (Shantaram S.); Wu, AI.G. (AI G.); Verbik, D.J. (David J.); et al. "Oligonucleotides complementary to c-myb messenger RNA inhibit growth and induce apoptosis in human Burkitt lymphoma cells". International journal of oncology. 8, 1996, 815 - 8201019-6439https://hdl.handle.net/10171/68915A 24-mer (antisense) phosphorothioate oligonucleotide (ODN) corresponding to the codons 2-9 of the c-myb gene was evaluated for its effects on the growth of a human Burkitt lymphoma cell line (Raji) in vitro. Raji cells incubated with different concentrations of c-myb antisense ODN (5-15 M-g/ml) for 24-72 h showed a significant dosedependent decrease in growth. The same concentrations of control (sense) or scrambled c-myb phosphorothioate ODNs did not inhibit Raji cell growth. The c-myb antisense ODN, but not the control ODNs, significantly decreased c-myb mRNA levels in treated cells as determined by RT-PCR. Additionally, the c-myb antisense ODN induced apoptosis of Raji cells as demonstrated by i) flow cytometry to enumerate the A0 (apoptotic cell population) population of propidium iodide stained cells; ii) electron microscopy to evaluate the cell morphology; and iii) DNA fragmentation pattern. Thus, an antisense c-myb ODN causes significant growth inhibition of Burkitt lymphoma cells, and one mechanism of growth inhibition is the induction of apoptosis of the lymphoma cells. In addition, antisense c-myb ODN did not reduce CFUGM or BFU-e colony-forming ability of normal hematopoietic stem/progenitor cells. Because the inhibition is sequence-specific and Burkitt lymphoma cell selective, evaluation of the therapeutic effects of c-myb antisense ODN against Burkitt lymphoma is warranted.enginfo:eu-repo/semantics/openAccessBurkitt lymphomaRaji cellsc-mybAntisense oligonucleotidesApoptosisinfo:eu-repo/semantics/article