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Dadun > Depósito Académico > Facultad de Ciencias > Departamento de Histología y Anatomía Patológica > DA - Ciencias - HAP - Artículos de revista >

Intracellular killing of Brucella melitensis in human macrophages with microspheres-encapsulated gentamicin
Autor(es) : Lecaroz, M.C. (María Concepción)
Burrell, M.A. (M.A.)
Blanco-Prieto, M.J. (María José)
Gamazo, C. (Carlos)
Palabras clave : Microparticles
Brucellosis
Phagocytosis
Drug delivery system
Fecha incorporación: 26-jun-2006
Editorial : Oxford University Press
Versión del editor: http://dx.doi.org/10.1093/jac/dkl257
ISSN: 0305-7453
Cita: Lecaroz C, Blanco-Prieto MJ, Burrell MA, Gamazo C. Intracellular killing of Brucella melitensis in human macrophages with microsphere-encapsulated gentamicin. J Antimicrob Chemother 2006 Sep;58(3):549-556.
Resumen
Objectives: Treatment of human brucellosis demands antibiotic targeting into the mononuclearphagocytic system. The aim of this work was to prepare and characterize particulate carriers containing gentamicin and to study their interactions with phagocytic cells and bactericidal activity against intracellular Brucella melitensis. Methods: Different poly(lactide-co-glycolide) (PLGA)polymers with free carboxylic end-group wereusedto formulate micro- and nanoparticles containing gentamicin, by a water-oil-water solvent-evaporation technique. PLGA 502H and 75:25H microparticles were selected because they showed the highest gentamicin loadings as well as good physico-chemical properties and sustained release in vitro. Results: Gentamicin-containing microspheres of both polymers were successfully phagocytosed by infected THP-1 human monocytes, and immunocytochemistry studies revealed that the antibiotic reached Brucella-specific compartments. A dose of 30 mg of encapsulated gentamicin was able to reduce intracellular Brucella infection by 2.2 log. Conclusions: Altogether, these results suggest that 502H and 75:25H microspheres are suitable carriers for gentamicin targeting inside human macrophages and thus for brucellosis treatment.
Enlace permanente: http://hdl.handle.net/10171/12440
Aparece en las colecciones: DA - Ciencias - HAP - Artículos de revista
DA - Medicina - Microbiología y Parasitología - Artículos de revista
DA - Farmacia - Tecnología Farmacéutica - Artículos de revista

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