Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Fortuño, A. (Ana) | - |
dc.creator | Muñiz, P. (Paula) | - |
dc.creator | Ravassa, S. (Susana) | - |
dc.creator | Rodriguez, J.A. (José Antonio) | - |
dc.creator | Fortuño, M.A. (María Antonia) | - |
dc.creator | Zalba, G. (Guillermo) | - |
dc.creator | Diez-Martinez, J. (Javier) | - |
dc.date.accessioned | 2011-04-20T14:15:50Z | - |
dc.date.available | 2011-04-20T14:15:50Z | - |
dc.date.issued | 1999 | - |
dc.identifier.citation | Fortuño A, Muñiz P, Ravassa S, Rodríguez JA, Fortuño MA, Zalba G, et al. Torasemide inhibits angiotensin II-induced vasoconstriction and intracellular calcium increase in the aorta of spontaneously hypertensive rats. Hypertension 1999 Jul;34(1):138-143. | es_ES |
dc.identifier.issn | 1524-4563 | - |
dc.identifier.uri | https://hdl.handle.net/10171/17842 | - |
dc.description.abstract | Torasemide is a loop diuretic that is effective at low once-daily doses in the treatment of arterial hypertension. Because its antihypertensive mechanism of action may not be based entirely on the elimination of salt and water from the body, a vasodilator effect of this drug can be considered. In the present study, the ability of different concentrations of torasemide to modify angiotensin II (Ang II)-induced vascular responses was examined, with the use of an organ bath system, in endothelium-denuded aortic rings from spontaneously hypertensive rats. Ang II-induced increases of intracellular free calcium concentration ([Ca(2+)](i)) were also examined by image analysis in cultured vascular smooth muscle cells (VSMCs) from spontaneously hypertensive rats. A dose-response curve to Ang II was plotted for cumulative concentrations (from 10(-9) to 10(-6) mol/L) in endothelium-denuded aortic rings (pD(2)=7.5+/-0.3). Isometric contraction induced by a submaximal concentration of Ang II (10(-7) mol/L) was reduced in a dose-dependent way by torasemide (IC(50)=0.5+/-0.04 micromol/L). Incubation of VSMCs with different concentrations of Ang II (from 10(-10) to 10(-6) mol/L) resulted in a dose-dependent rise of [Ca(2+)](i) (pD(2)=7.5+/-0.3). The stimulatory effect of [Ca(2+)](i) induced by a submaximal concentration of Ang II (10(-7) mol/L) was blocked by torasemide (IC(50)=0.5+/-0.3 nmol/L). Our findings suggest that torasemide blocks the vasoconstrictor action of Ang II in vitro. This action can be related to the ability of torasemide to block the increase of [Ca(2+)](i) induced by Ang II in VSMCs. It is proposed that these actions might be involved in the antihypertensive effect of torasemide observed in vivo. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | American Heart Association | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Angiotensin II | es_ES |
dc.subject | Calcium | es_ES |
dc.subject | Furosemide | es_ES |
dc.subject | Irbesartan | es_ES |
dc.subject | Muscle, smooth, vascular | es_ES |
dc.subject | Rats, inbred SHR | es_ES |
dc.subject | Torasemide | es_ES |
dc.title | Torasemide inhibits angiotensin II-induced vasoconstriction and intracellular calcium increase in the aorta of spontaneously hypertensive rats | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://hyper.ahajournals.org/cgi/content/full/34/1/138 | es_ES |
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