Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Rodriguez, A. (Amaia) | - |
dc.creator | Gomez-Ambrosi, J. (Javier) | - |
dc.creator | Catalan, V. (Victoria) | - |
dc.creator | Fortuño, A. (Ana) | - |
dc.creator | Frühbeck, G. (Gema) | - |
dc.date.accessioned | 2011-05-13T16:27:07Z | - |
dc.date.available | 2011-05-13T16:27:07Z | - |
dc.date.issued | 2010 | - |
dc.identifier.citation | Rodriguez A, Gomez-Ambrosi J, Catalan V, Fortuño A, Fruhbeck G. Leptin inhibits the proliferation of vascular smooth muscle cells induced by angiotensin II through nitric oxide-dependent mechanisms. Mediators Inflamm 2010;2010:105489 | es_ES |
dc.identifier.issn | 1466-1861 | - |
dc.identifier.uri | https://hdl.handle.net/10171/17923 | - |
dc.description.abstract | OBJECTIVE: This study was designed to investigate whether leptin modifies angiotensin (Ang) II-induced proliferation of aortic vascular smooth muscle cells (VSMCs) from 10-week-old male Wistar and spontaneously hypertensive rats (SHR), and the possible role of nitric oxide (NO). METHODS: NO and NO synthase (NOS) activity were assessed by the Griess and (3)H-arginine/citrulline conversion assays, respectively. Inducible NOS (iNOS) and NADPH oxidase subutnit Nox2 expression was determined by Western-blot. The proliferative responses to Ang II were evaluated through enzymatic methods. RESULTS: Leptin inhibited the Ang II-induced proliferative response of VSMCs from control rats. This inhibitory effect of leptin was abolished by NOS inhibitor, NMMA, and iNOS selective inhibitor, L-NIL, and was not observed in leptin receptor-deficient fa/fa rats. SHR showed increased serum leptin concentrations and lipid peroxidation. Despite a similar leptin-induced iNOS up-regulation, VSMCs from SHR showed an impaired NOS activity and NO production induced by leptin, and an increased basal Nox2 expression. The inhibitory effect of leptin on Ang II-induced VSMC proliferation was attenuated. CONCLUSION: Leptin blocks the proliferative response to Ang II through NO-dependent mechanisms. The attenuation of this inhibitory effect of leptin in spontaneous hypertension appears to be due to a reduced NO bioavailability in VSMCs. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Hindawi Publishing Corporation | es_ES |
dc.rights | info:eu-repo/semantics/closedAccess | - |
dc.subject | Leptin | es_ES |
dc.subject | Angiotensin II | es_ES |
dc.subject | Nitric Oxide | es_ES |
dc.title | Leptin inhibits the proliferation of vascular smooth muscle cells induced by angiotensin II through nitric oxide-dependent mechanisms | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://www.hindawi.com/journals/mi/2010/105489/ | es_ES |
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