Full metadata record
DC Field | Value | Language |
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dc.creator | Martinez-Forero, I. (Iván) | - |
dc.creator | Garcia-Muñoz, R. (R.) | - |
dc.creator | Martinez-Pasamar, S. (Sara) | - |
dc.creator | Inoges, S. (Susana) | - |
dc.creator | Lopez-Diaz-de-Cerio, A. (Ascensión) | - |
dc.creator | Palacios, R. (Ricardo) | - |
dc.creator | Sepulcre, J. (Jorge) | - |
dc.creator | Moreno, B. (Beatriz) | - |
dc.creator | Gonzalez, Z. (Zaira) | - |
dc.creator | Fernandez-Diez, B. (B.) | - |
dc.creator | Melero, I. (Ignacio) | - |
dc.creator | Bendandi, M. (Maurizio) | - |
dc.creator | Villoslada, P. (Pablo) | - |
dc.date.accessioned | 2011-07-04T07:58:47Z | - |
dc.date.available | 2011-07-04T07:58:47Z | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | Martínez-Forero I, García-Muñoz R, Martínez-Pasamar S, Inoges S, López-Díaz de Cerio A, Palacios R, et al. IL-10 suppressor activity and ex vivo Tr1 cell function are impaired in multiple sclerosis. Eur J Immunol 2008 Feb;38(2):576-586. | es_ES |
dc.identifier.issn | 1521-4141 | - |
dc.identifier.uri | https://hdl.handle.net/10171/18714 | - |
dc.description.abstract | T regulatory cells type 1 (Tr1 cells) are excellent candidates for cell therapy in multiple sclerosis (MS). The aim of our study was to assess the functional state of Tr1 cells and IL-10R signaling in patients with MS. Tr1 cells were induced in vitro by activation with anti-CD46 antibodies in controls and patients with MS. Cells were phenotyped by cytometry and suppression assays, and the expression of cytokines and transcription factors was evaluated by real-time PCR, ELISA, cytometry and Western blotting. We found that the activity of Tr1 cells and IL-10R signaling is impaired in MS patients since Tr1 cells isolated from MS patients produced less IL-10 than those obtained from controls. Indeed, the supernatants from Tr1 cells from controls did not suppress the proliferation of stimulated CD4(+) cells from patients with MS. Furthermore, the IL-10R signaling pathway was not fully active in CD4(+) cells from MS patients and these cells had higher baseline levels of SOCS3 transcripts than controls. Indeed, after in vitro IL-10 stimulation, the expression levels of the STAT1, STAT3 and IL-10RA genes were higher in MS patients than in controls. Moreover, Stat-3 phosphorylation was lower in controls than in patients after IL-10 stimulation. These results indicate that IL-10 regulatory function is impaired in patients with MS. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Wiley-Blackwell | es_ES |
dc.rights | info:eu-repo/semantics/closedAccess | - |
dc.subject | CD46 | es_ES |
dc.subject | IL-10 | es_ES |
dc.subject | MS | es_ES |
dc.subject | Stat-3 | es_ES |
dc.subject | Tr1 cells Abbreviations: c(t): cycle threshold HC: healthy control MNE: mean normalized expression MS: multiple sclerosis Socs: suppressor of cytokine signaling Tr1 cell: T regulatory cell type 1 576 Ivan Martinez-Forero et al. Eur. J. Immunol. 2008. 38: 576–586 f 2008 | es_ES |
dc.title | IL-10 suppressor activity and ex vivo Tr1 cell function are impaired in multiple sclerosis | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://onlinelibrary.wiley.com/doi/10.1002/eji.200737271/abstract | es_ES |
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