Immunomodulation induced by synthetic peptides derived from Staphylococcus aureus protein A
Palabras clave : 
Immunomodulatory peptide
Cytotoxicity
Tumor necrosis factor alfa
Interleukin 1
Lymphomononuclear cell
Monocyte
Fecha de publicación : 
1994
Editorial : 
Elsevier
ISSN : 
0167-4889
Cita: 
Lopez-Moratalla N, Lopez-Zabalza MJ, Subira ML, Borras-Cuesta F, Perez-Mediavilla A, Santiago E. Immunomodulation induced by synthetic peptides derived from Staphylococcus aureus protein A. Biochim Biophys Acta. 1994 Mar 31;1221(2):153-158.
Resumen
Peptides from 10 to 22 amino acids containing sequences encompassed by Staphylococcus aureus protein A were synthesized. Some of these peptides, when present in cultures of lymphomononuclear cells from healthy donors or from cancer patients (melanoma, breast carcinoma, non-Hodgkin lymphoma and renal cell carcinoma) promoted: (i) changes in the phenotype of the lymphomononuclear population, (ii) stimulation of monocytes (release of IL-1 and TNF-alpha), and (iii) an increase in cytotoxicity against K562, Daudi and HT-29 cells. Isolated monocytes responded also to those peptides with a release of IL-1 and TNF alpha and an increase of cytotoxicity against HT-29 cells. It was found that the active peptides had the following structural pattern: a length of at least 15 amino-acid residues with a proline at position 6, valine, leucine, isoleucine, glycine, alanine or lysine at position 2, and glutamic or aspartic acid at position 11. Replacement of Pro at position 6 with any other residue turned the peptide inactive. Replacement of residues at positions 2 and 11 with amino-acid residues other than those required for activity resulted in compounds with a marked decrease in the immunomodulating properties described, or lacking these properties altogether.

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