Full metadata record
DC Field | Value | Language |
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dc.creator | Montero, I. (Inés) | - |
dc.creator | Orbe, J. (Josune) | - |
dc.creator | Varo-Cenarruzabeitia, M.N. (Miren Nerea) | - |
dc.creator | Beloqui, O. (Óscar) | - |
dc.creator | Monreal, J.I. (José Ignacio) | - |
dc.creator | Rodriguez, J.A. (José Antonio) | - |
dc.creator | Diez-Martinez, J. (Javier) | - |
dc.creator | Libby, P. (Peter) | - |
dc.creator | Paramo, J.A. (José Antonio) | - |
dc.date.accessioned | 2012-05-02T12:17:32Z | - |
dc.date.available | 2012-05-02T12:17:32Z | - |
dc.date.issued | 2006 | - |
dc.identifier.citation | Montero I, Orbe J, Varo N, Beloqui O, Monreal JI, Rodriguez JA, et al. C-reactive protein induces matrix metalloproteinase-1 and -10 in human endothelial cells: implications for clinical and subclinical atherosclerosis. J Am Coll Cardiol 2006 Apr 4;47(7):1369-1378. | es_ES |
dc.identifier.issn | 1558-3597 | - |
dc.identifier.uri | https://hdl.handle.net/10171/21870 | - |
dc.description.abstract | OBJECTIVES: We examined the effect of C-reactive protein (CRP) on matrix metalloproteinase (MMP) and inhibitor expression in endothelial cells and in patients with clinical and subclinical atherosclerosis. BACKGROUND: In addition to predicting atherosclerotic vascular disease, CRP may directly promote a proinflammatory/proatherosclerotic phenotype. METHODS: Human umbilical vein endothelial cells (HUVECs) and aortic endothelial cells (HAECs) were incubated in the presence or absence of CRP (50 mug/ml). Microarray analysis, real-time polymerase chain reaction, immunological and activity assays for MMPs were performed. Specific inhibitors of mitogen-activated protein kinase pathway were used. The MMP-1 and -10 plasma levels were measured in apparently healthy subjects (n = 70). Immunolocalization of CRP, MMP-1, and MMP-10 was performed in human mammary arteries and carotid endarterectomy specimens. RESULTS: C-reactive protein augmented MMP-1 and -10 messenger ribonucleic acid expression in HUVEC (p < 0.05) and HAEC (p < 0.01). C-reactive protein stimulation also increased MMP-1 and -10 protein in conditioned culture medium (p < 0.001), as well as MMP activity (p = 0.001). Specific inhibition of p38 or MEK abolished the CRP induction of the MMP-1, whereas MMP-10 induction blockade required the simultaneous inhibition of p38 and Jun N-terminal kinase pathways. Subjects with CRP values >3 mg/l (n = 37) had increased plasma MMP-1 and -10 (p < 0.05), the association being significant after adjustment for confounding variables (p = 0.04 and p = 0.008, respectively). The MMP-10 levels were elevated in subjects with higher carotid intima-media thickness (p = 0.009). Increased CRP and MMP-10 colocalized in endothelial layer and macrophage-rich areas in advanced atherosclerotic plaques. CONCLUSIONS: Increased local and systemic CRP-related MMP activation might provide a link between inflammation and plaque vulnerability. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Atherosclerosis/metabolism | es_ES |
dc.subject | C-Reactive Protein/physiology | es_ES |
dc.subject | Endothelial Cells/enzymology | es_ES |
dc.subject | Matrix Metalloproteinase 1/biosynthesis | es_ES |
dc.subject | Metalloendopeptidases/biosynthesis | es_ES |
dc.title | C-reactive protein induces matrix metalloproteinase-1 and -10 in human endothelial cells: implications for clinical and subclinical atherosclerosis | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S0735109706001513 | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
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