Full metadata record
DC FieldValueLanguage
dc.creatorParra-Guillen, Z.P. (Zinnia Patricia)-
dc.creatorFioravanti, J. (Jessica)-
dc.creatorMedina-Echeverz, J. (José)-
dc.creatorGomer, C. (Celia)-
dc.creatorArdaiz, N. (Nuria)-
dc.creatorTroconiz, I.F. (Iñaki F.)-
dc.creatorBerraondo, P. (Pedro)-
dc.date.accessioned2012-10-09T08:28:41Z-
dc.date.available2012-10-09T08:28:41Z-
dc.date.issued2012-
dc.identifier.citationParra-Guillen ZP, Fioravanti J, Medina-Echeverz J, Gomar C, Ardaiz N, Troconiz IF, et al. Kinetic and dynamic computational model-based characterization of new proteins in mice: application to interferon alpha linked to apolipoprotein a-I. PLoS One 2012;7(7):e42100.es_ES
dc.identifier.issn1932-6203-
dc.identifier.urihttp://hdl.handle.net/10171/23326-
dc.description.abstractInterferon alpha linked to apolipoprotein A-I has been recently proposed as an improved interferon-based therapy. In the present study, we aimed to develop a computational model to gain further insight into the in vivo behaviour of this new fusion protein. In order to facilitate in vivo evaluation of interferon and the fusion protein without altering their biological properties, green fluorescent protein was incorporated into their structures. Kinetic and dynamic behaviour of both compounds was successfully described after plasmid hydrodynamic administration and in situ synthesis of the studied proteins. Results from the modelling exercise showed that apolipoprotein A-I conferred a modified kinetic behaviour, varying molecule distribution and prolonging half-life without altering liver dynamic performance. However, differences in the gene expression activity were observed at brain level between both compounds. Those differences could be explained by modifications in the dynamic, but also in the biodistribution properties, which would be worth evaluating in future experiments. Therefore, the modelling approach provided a global comprehension of a complex system and allowed us to compare the in vivo behaviour of both compounds and to identify critical aspects that might be important to understand the system better and suggests a need for new model-based experiments.es_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Sciencees_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectInterferon Alphaes_ES
dc.subjectApolipoprotein A-Ies_ES
dc.titleKinetic and dynamic computational model-based characterization of new proteins in mice: application to interferon alpha linked to apolipoprotein a-Ies_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0042100es_ES
dc.type.driverinfo:eu-repo/semantics/articlees_ES

Files in This Item:
File: 
PlosOne2012_7_7_e42100.pdf
Description: 
Size: 
471,55 kB
Format: 
Adobe PDF


Items in Dadun are protected by copyright, with all rights reserved, unless otherwise indicated.