Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR

Romero, C. (Conchi); Lopez-Goñi, I. (Ignacio)

Autor(es):

Romero, C. (Conchi)

Lopez-Goñi, I. (Ignacio)

Palabras clave :

Blood
RNA
Biotechnology and applied microbiology
Microbiology

Fecha de publicación :

1999

Editorial :

American Society for Microbiology

ISSN :

0099-2240

Cita:

Romero C, Lopez-Goni I. Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR. Appl Environ Microbiol 1999 Aug;65(8):3735-3737.

Resumen

Different methods of extraction of bacterial DNA from bovine milk to improve the direct detection of Brucella by PCR were evaluated. We found that the use of a lysis buffer with high concentrations of Tris, EDTA, and NaCl, high concentrations of sodium dodecyl sulfate and proteinase K, and high temperatures of incubation was necessary for the efficient extraction of Brucella DNA. The limit of detection by PCR was 5 to 50 Brucella CFU/ml of milk.

URI :

https://hdl.handle.net/10171/29637

Aparece en las colecciones:

DA - Medicina - Microbiología y Parasitología - Artículos de revista

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