Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Tamames-Tabar, C. (Cristina) | - |
dc.creator | Imbuluzqueta, E. (Edurne) | - |
dc.creator | Campanero, M.A. (Miguel Angel) | - |
dc.creator | Horcajada, P. (Patricia) | - |
dc.creator | Blanco-Prieto, M.J. (María José) | - |
dc.date.accessioned | 2013-12-23T15:25:22Z | - |
dc.date.available | 2013-12-23T15:25:22Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Tamames-Tabar C, Imbuluzqueta E, Campanero MA, Horcajada P, Blanco-Prieto MJ. A simple and robust high-performance liquid chromatography coupled to a diode-array detector method for the analysis of genistein in mouse tissues. J Chromatogr B 2013 SEP 15;935:47-53 | es_ES |
dc.identifier.issn | 1570-0232 | - |
dc.identifier.uri | https://hdl.handle.net/10171/34790 | - |
dc.description.abstract | A simple liquid-liquid extraction procedure and quantification by high-performance liquid chromatography (HPLC) method coupled to a diode-array detector (DAD) of genistein (GEN) was developed in various mouse biological matrices. 7-ethoxycoumarin was used as internal standard (IS) and peaks were optimally separated using a Kinetex C18 column (2.6 µm, 150 mm X 2.10 mm I.D.) at 40 ºC with an isocratic elution of mobile phase with sodium dihydrogen phosphate 0.01M in water at pH 2.5 and methanol (55:45, v/v), at a flow rate of 0.25 mL/min. The injection volume was 10 µL. In all cases, the range of GEN recovery was higher than 61%. The low limit of quantification (LLOQ) was 25 ng/mL. The linearity of the calibration curves was satisfactory in all cases as shown by correlation coefficients >0.996. The within-day and between-day precisions were <15% and the accuracy ranged in all cases between 90.14 and 106.05%. This method was successfully applied to quantify GEN in liver, spleen, kidney and plasma after intravenous administration of a single dose (30 mg/Kg) in female BALB/C mice. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Genistein | es_ES |
dc.subject | HPLC | es_ES |
dc.subject | Extraction method | es_ES |
dc.subject | Biological matrices | es_ES |
dc.subject | Method validation | es_ES |
dc.title | A simple and robust high-performance liquid chromatography coupled to a diode-array detector method for the analysis of genistein in mouse tissues | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | http://dx.doi.org/10.1016/j.jchromb.2013.07.020 | - |
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