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Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.creator | Rosa, S. (S.) | - |
dc.creator | Praça, C. (C.) | - |
dc.creator | Pitrez, P.R. (P. R.) | - |
dc.creator | Gouveia, P.J. (P. José) | - |
dc.creator | Aranguren, X.L. (Xabier L.) | - |
dc.creator | Ricotti, L. (L.) | - |
dc.creator | Ferreira, L.S. (L. Silva) | - |
dc.date.accessioned | 2021-10-04T10:51:12Z | - |
dc.date.available | 2021-10-04T10:51:12Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Rosa, S. (S.); Praça, C. (C.); Pitrez, P.R. (P. R.); et al. "Functional characterization of iPSC-derived arterial- and venous-like endothelial cells". Scientific Reports. 9 (3826), 2019, 1 - 15 | es_ES |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.other | PMID: 30846769 | - |
dc.identifier.uri | https://hdl.handle.net/10171/62142 | - |
dc.description.abstract | The current work reports the functional characterization of human induced pluripotent stem cells (iPSCs)- arterial and venous-like endothelial cells (ECs), derived in chemically defined conditions, either in monoculture or seeded in a scaffold with mechanical properties similar to blood vessels. iPSC-derived arterial- and venous-like endothelial cells were obtained in two steps: differentiation of iPSCs into endothelial precursor cells (CD31pos/KDRpos/VE-Cadmed/EphB2neg/COUP-TFneg) followed by their differentiation into arterial and venous-like ECs using a high and low vascular endothelial growth factor (VEGF) concentration. Cells were characterized at gene, protein and functional levels. Functionally, both arterial and venous-like iPSC-derived ECs responded to vasoactive agonists such as thrombin and prostaglandin E2 (PGE2), similar to somatic ECs; however, arterial-like iPSC-derived ECs produced higher nitric oxide (NO) and elongation to shear stress than venous-like iPSC-derived ECs. Both cells adhered, proliferated and prevented platelet activation when seeded in poly(caprolactone) scaffolds. Interestingly, both iPSC-derived ECs cultured in monoculture or in a scaffold showed a different inflammatory profile than somatic ECs. Although both somatic and iPSC-derived ECs responded to tumor necrosis factor-α (TNF-α) by an increase in the expression of intercellular adhesion molecule 1 (ICAM-1), only somatic ECs showed an upregulation in the expression of E-selectin or vascular cell adhesion molecule 1 (VCAM-1). | es_ES |
dc.description.sponsorship | Tis work was supported by FEDER through the Program COMPETE and by Portuguese fund through FCT in context of the project MITP TB/ECE/0013/2013. Te work was also funded by a ERA Chair project ERA@ UC (ref: 669088) through European Union’s Horizon 2020 program and project NETDIAMOND: new targets in diastolic heart failure, from comorbidities to personalized medicine” (Ref: POCI-01-0145-FEDER-016385), Funding agency: Portugal 2020. SR wishes to thank FCT for a BPD fellowship (SFRH/BPD/79232/2011). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Springer Science and Business Media LLC | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/952266/EU | es_ES |
dc.relation | info:eu-repo/grantAgreement/FCT/5665-PICT/137185/PT | - |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/669088/EU | - |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Materias Investigacion::Ciencias de la Salud::Hematología | es_ES |
dc.title | Functional characterization of iPSC-derived arterial- and venous-like endothelial cells | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.description.note | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made | es_ES |
dc.identifier.doi | 10.1038/s41598-019-40417-9 | - |
dadun.citation.endingPage | 15 | es_ES |
dadun.citation.number | 3826 | es_ES |
dadun.citation.publicationName | Scientific Reports | es_ES |
dadun.citation.startingPage | 1 | es_ES |
dadun.citation.volume | 9 | es_ES |
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