Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | San-Jose, G. (Gorka) | |
dc.creator | Bidegain, J. (J.) | |
dc.creator | Robador, P.A. (Pablo A.) | |
dc.creator | Diez-Martinez, J. (Javier) | |
dc.creator | Fortuño, A. (Ana) | |
dc.creator | Zalba, G. (Guillermo) | |
dc.date.accessioned | 2011-05-19T08:19:06Z | - |
dc.date.available | 2011-05-19T08:19:06Z | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | San Jose G, Bidegain J, Robador PA, Diez J, Fortuño A, Zalba G. Insulin-induced NADPH oxidase activation promotes proliferation and matrix metalloproteinase activation in monocytes/macrophages. Free Radic Biol Med 2009 Apr 15;46(8):1058-1067. | es_ES |
dc.identifier.issn | 1873-4596 | - |
dc.identifier.uri | https://hdl.handle.net/10171/18011 | - |
dc.description.abstract | Insulin stimulates superoxide (O(2)(-)) production in monocytes and macrophages. However, the mechanisms through which insulin induces O(2)(-) production are not completely understood. In this study, we (a) characterized the enzyme and the pathways involved in insulin-stimulated O(2)(-) production in human monocytes and murine macrophages, and (b) analyzed the consequences of insulin-stimulated O(2)(-) production on the cellular phenotype in these cells. We showed that insulin stimulated O(2)(-) production, and promoted p47(phox) translocation to the plasma membrane. Insulin-induced O(2)(-) production and p47(phox) translocation were prevented in the presence of specific inhibitors of PI3K and PKC. Insulin-mediated NADPH oxidase activation stimulated MMP-9 activation in monocytes and cell proliferation in macrophages. The effect of insulin on these phenotypic responses was mediated through NFkappaB, p38MAPK, and ERK 1/2 activation. Small-interfering RNA-specific gene silencing targeted specifically against Nox2 reduced the cognate protein expression, decreased insulin-induced O(2)(-) production, inhibited the turn on of NFkappaB, p38MAPK, and ERK 1/2, and reduced cell proliferation in macrophages. These findings suggest a pivotal role for NADPH oxidase in insulin-induced proliferation and proteolytic activation in monocytes and macrophages, respectively, and identify a pathway that may play a pathological role in hyperinsulinemic states. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | info:eu-repo/semantics/closedAccess | - |
dc.subject | Insulin | es_ES |
dc.subject | Monocyte/macrophage | es_ES |
dc.subject | NADPH oxidase | es_ES |
dc.subject | Superoxide | es_ES |
dc.subject | Matrix metalloproteinase | es_ES |
dc.title | Insulin-induced NADPH oxidase activation promotes proliferation and matrix metalloproteinase activation in monocytes/macrophages | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S0891584909000185 | es_ES |
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