Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Ochoa, L. (Laura) | - |
dc.creator | Otano, I. (Itziar) | - |
dc.creator | Vales, A. (África) | - |
dc.creator | Olagüe, C. (Cristina) | - |
dc.creator | Sarobe, P. (Pablo) | - |
dc.creator | Lasarte, J.J. (Juan José) | - |
dc.creator | Menne, S. (Stephan) | - |
dc.creator | Prieto, J. (Jesús) | - |
dc.creator | González-Aseguinolaza, G. (Gloria) | - |
dc.date.accessioned | 2011-12-23T13:36:15Z | - |
dc.date.available | 2011-12-23T13:36:15Z | - |
dc.date.issued | 2010 | - |
dc.identifier.citation | Ochoa-Callejero L, Otano I, Vales A, Olagüe C, Sarobe P, Lasarte JJ, et al. Identification of CD4+ and CD8+ T cell epitopes of woodchuck hepatitis virus core and surface antigens in BALB/c mice. Vaccine 2010 Jul 19;28(32):5323-5331. | es_ES |
dc.identifier.issn | 0264-410X, | - |
dc.identifier.uri | https://hdl.handle.net/10171/20365 | - |
dc.description.abstract | A therapeutic vaccine against chronic hepatitis B virus (HBV) infection requires the development of a strong and multispecific Th1 cell immune response. Woodchucks chronically infected with the woodchuck hepatitis virus (WHV) closely resemble HBV infection and represent the best animal model for this hepadnavirus-induced disease. Using the BIMAS "HLA Peptide Binding Predictions" program, we have identified and further characterized novel H-2(d)-restricted CD8+ epitopes within the WHV core (peptides C#12-21, C#18-32, C#19-27, C#61-69) and surface antigens (peptides preS2#10-18, preS2#27-35, S#76-84, S#133-140 and S#257-265), respectively. These peptides bind to H-2(d) with high efficiency and upon immunization of mice with peptide and Freund's adjuvant they induce the development of IFN-gamma producing T cells. More importantly, WHV core peptides C#19-27 and C#61-69 and WHV surface peptides S#133-140 and S#257-265 were also recognized by CD8+ T cells after immunization of mice with DNA/PEI nanoparticles. Direct stimulation of splenocytes obtained from such DNA-immunized mice with peptides C#18-32, S#76-84, and S#257-265 resulted in significant production of IFN-gamma. Thus, we have identified T cell determinants in mice from WHV core and surface antigens that have important value for designing and evaluating an effective vaccine against hepadnavirus infection. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | info:eu-repo/semantics/closedAccess | - |
dc.subject | Woodchuck hepatitis virus | es_ES |
dc.subject | T cell epitopes | es_ES |
dc.subject | Hepatitis B virus | es_ES |
dc.subject | Chronic hepatitis infection | es_ES |
dc.subject | DNA vaccine | es_ES |
dc.title | Identification of CD4+ and CD8+ T cell epitopes of woodchuck hepatitis virus core and surface antigens in BALB/c mice | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | http://dx.doi.org/10.1016/j.vaccine.2010.05.043 | es_ES |
Files in This Item:
There are no files associated with this item.
Statistics and impact
Items in Dadun are protected by copyright, with all rights reserved, unless otherwise indicated.