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dc.creatorRodriguez-Madoz, J.R. (Juan Roberto)-
dc.creatorPrieto, J. (Jesús)-
dc.creatorSmerdou, C. (Cristian)-
dc.date.accessioned2012-09-25T10:42:47Z-
dc.date.available2012-09-25T10:42:47Z-
dc.date.issued2005-
dc.identifier.citationRodriguez-Madoz JR, Prieto J, Smerdou C. Semliki forest virus vectors engineered to express higher IL-12 levels induce efficient elimination of murine colon adenocarcinomas. Mol Ther 2005 Jul;12(1):153-163.es_ES
dc.identifier.issn1525-0024-
dc.identifier.urihttps://hdl.handle.net/10171/23177-
dc.description.abstractTo evaluate the use of alphavirus vectors for tumor treatment we have constructed and compared two Semliki Forest virus (SFV) vectors expressing different levels of IL-12. SFV-IL-12 expresses both IL-12 subunits from a single subgenomic promoter, while in SFV-enhIL-12 each IL-12 subunit is expressed from an independent subgenomic promoter fused to the SFV capsid translation enhancer. This latter strategy provided an eightfold increase of IL-12 expression. We chose the poorly immunogenic MC38 colon adenocarcinoma model to evaluate the therapeutic potential of SFV vectors. A single intratumoral injection of 10(8) viral particles of SFV-IL-12 or SFV-enh-IL-12 induced>or=80% complete tumor regressions with long-term tumor-free survival. However, lower doses of SFV-enhIL-12 were more efficient than SFV-IL-12 in inducing antitumoral responses, indicating a positive correlation between the IL-12 expression level and the therapeutic effect. Moreover, repeated intratumoral injections of suboptimal doses of SFV-enhIL-12 increased the antitumoral response. In all cases SFV vectors were more efficient at eliminating tumors than a first-generation adenovirus vector expressing IL-12. In addition, the antitumoral effect of SFV vectors was only moderately affected by preimmunization of animals with high doses of SFV vectors. This antitumoral effect was produced, at least partially, by a potent CTL-mediated immune response.es_ES
dc.language.isoenges_ES
dc.publisherNaturees_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectSemliki Forest virus vectorses_ES
dc.subjectSFV translation enhanceres_ES
dc.subjectGene therapyes_ES
dc.subjectMC38es_ES
dc.subjectColon adenocarcinomaes_ES
dc.subjectIL-12es_ES
dc.titleSemliki forest virus vectors engineered to express higher IL-12 levels induce efficient elimination of murine colon adenocarcinomases_ES
dc.typeArticuloes_ES
dc.relation.publisherversionhttp://www.nature.com/mt/journal/v12/n1/full/mt20051278a.htmles_ES
dc.type.driverinfo:eu-repo/semantics/articlees_ES

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