Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Qian, C. (Cheng) | - |
dc.creator | Diez-Martinez, J. (Javier) | - |
dc.creator | Larrea, E. (Esther) | - |
dc.creator | Garciandia, A. (Ana) | - |
dc.creator | Arrazola, A. (Arantxa) | - |
dc.creator | Civeira, M.P. (María Pilar) | - |
dc.creator | Medina, J.F. (Juan Francisco) | - |
dc.creator | Prieto, J. (Jesús) | - |
dc.date.accessioned | 2012-10-08T10:32:50Z | - |
dc.date.available | 2012-10-08T10:32:50Z | - |
dc.date.issued | 1992 | - |
dc.identifier.citation | Qian C, Diez J, Larrea E, Garciandia A, Arrazola A, Civeira MP, et al. The stilbene disulfonic acid DIDS stimulates the production of TNF-alpha in human lymphocytes. Biochem Biophys Res Commun 1992 Dec 30;189(3):1268-1274. | es_ES |
dc.identifier.issn | 1090-2104 | - |
dc.identifier.uri | https://hdl.handle.net/10171/23321 | - |
dc.description.abstract | Exposure of human peripheral blood mononuclear cells (PBMC) to the stilbene derivative DIDS (4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid) (60 microM and above) significantly increased the release of tumor necrosis factor-alpha (TNF-alpha), as determined by TNF-alpha activity in the incubation media. When the TNF-alpha message was analyzed in PBMC by a reverse transcription/polymerase chain reaction (RT/PCR)-based procedure, it was found that incubation with DIDS (60 microM) was followed by a time-dependent accumulation of TNF-alpha mRNA. Measurements of intracellular pH showed that the presence of increasing concentrations of DIDS resulted in a progressive intracellular alkalinization of PBMC. It is suggested that the known DIDS effect of inhibiting transmembrane anion exchange, i.e., chloride/bicarbonate exchange, might play a role in the stimulation of TNF-alpha production by PBMC exposed to DIDS. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid/analogs & derivatives | es_ES |
dc.subject | Lymphocytes/drug effects | es_ES |
dc.subject | Tumor Necrosis Factor-alpha/biosynthesis | es_ES |
dc.title | The stilbene disulfonic acid DIDS stimulates the production of TNF-alpha in human lymphocytes | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/0006291X9290210C | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
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