Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Menendez-Carreño, M. (María) | - |
dc.creator | Garcia-Herreros, C. (Cecilia) | - |
dc.creator | Ansorena-Artieda, D. (Diana) | - |
dc.creator | Astiasarán, I. (Iciar) | - |
dc.date.accessioned | 2012-05-18T18:03:14Z | - |
dc.date.available | 2012-05-18T18:03:14Z | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | Menendez-Carreno M, Garcia-Herreros C, Astiasaran I, Ansorena D. Validation of a gas chromatography-mass spectrometry method for the analysis of sterol oxidation products in serum. J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Mar 15;864(1-2):61-68. | es_ES |
dc.identifier.issn | 1570-0232 | - |
dc.identifier.uri | https://hdl.handle.net/10171/22124 | - |
dc.description.abstract | A validated gas chromatography-mass spectrometry (GC-MS) detection method for the quantitative analysis of sterol oxidation products (SOPs) in serum is described. After a lipid extraction procedure with chloroform-methanol, a cold saponification and purification by solid phase extraction, oxysterols were derivatized to form trimethyl-sylil-ethers which were subjected to GC-MS analysis. Calibration curves for cholesterol oxidation products showed determination coefficient (R(2)) of 1.0, with low values for the coefficient of variation of the response factors (< 1%). Detection and quantification limits were below 5 ng/mL and 10 ng/mL, respectively. Recovery data were between 77.65% and 110.29% (CV < 10% for all compounds). Good results were obtained for within- and between-day repeatability, with values below 10%. In conclusion, the method performed is suitable for the determination and quantification of SOPs in serum. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Serum | es_ES |
dc.subject | Sterols | es_ES |
dc.subject | COPs | es_ES |
dc.subject | Linearity | es_ES |
dc.subject | Recovery | es_ES |
dc.subject | Repeatability | es_ES |
dc.title | Validation of a gas chromatography-mass spectrometry method for the analysis of sterol oxidation products in serum | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | http://dx.doi.org/10.1016/j.jchromb.2008.01.036 | es_ES |
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