Some cyclin-dependent kinase inhibitors-related genes are regulated by vitamin C in a model of diet-induced obesity
Keywords: 
Cyclin-dependent kinase inhibitor
Cafeteria diet
Ascorbic acid
Adipose tissue
Fatty liver
Issue Date: 
2009
Publisher: 
Pharmaceutical Society of Japan
ISSN: 
0918-6158
Citation: 
Boque N, Campion J, Milagro FI, Moreno-Aliaga MJ, Martinez JA. Some cyclin-dependent kinase inhibitors-related genes are regulated by vitamin C in a model of diet-induced obesity. Biol Pharm Bull 2009 Aug;32(8):1462-1468
Abstract
The aim of this research was to investigate differential gene expression of cyclin-dependent kinase inhibitors (CKIs) in white adipose tissue (WAT) and liver from high-fat fed male Wistar rats with or without vitamin C (VC) supplementation (750 mg/kg of body weight). After 56 d of experimentation, animals fed on a cafeteria diet increased significantly body weights and total body fat. Reverse transcription-polymerase chain reaction (RT-PCR) studies showed that cafeteria diet decreased p21 and p57 mRNA expression in subcutaneous WAT and increased p21 mRNA in liver. Overall, these data provide new information about the role of high fat intake on mRNA levels of several CKIs with implications in adipogenesis, cell metabolism and weight homeostasis. Interestingly, VC supplementation partially prevented diet-induced adiposity and increased p27 mRNA in liver without any changes in the other tissues and genes analyzed. Thus, hepatic mRNA changes induced by ascorbic acid indicate a possible role of these genes in diet-induced oxidative stress processes.

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