Effect of TNF-Alpha on caveolin-1 expression and insulin signaling during adipocyte differentiation and in mature adipocytes
dc.contributor.author | Martinez, J.A. (José Alfredo) | |
dc.contributor.author | Algarabel, M. (Miriam) | |
dc.contributor.author | Miguel-Vázquez, C. (Carlos) de | |
dc.contributor.author | Milagro-Yoldi, F.I. (Fermín Ignacio) | |
dc.contributor.author | Palacios-Ortega, S. (Sara) | |
dc.contributor.author | Varela-Guruceaga, M. (Maider) | |
dc.date.accessioned | 2015-09-21T16:12:29Z | |
dc.date.available | 2015-09-21T16:12:29Z | |
dc.date.issued | 2015 | |
dc.description.abstract | Background/Aims: Tumor necrosis factor-α (TNF-α)-mediated chronic low-grade inflammation of adipose tissue is associated with obesity and insulin resistance. Caveolin-1 (Cav-1) is the central component of adipocyte caveolae and has an essential role in the regulation of insulin signaling. The effects of TNF-α on Cav-1 expression and insulin signaling during adipocyte differentiation and in mature adipocytes were studied. Methods: 3T3-L1 cells were differentiated (21 days) in the presence TNF-α (10 ng/mL) and mature adipocytes were also treated with TNF-α for 48 hours. Cav-1 and insulin receptor (IR) gene methylation were determined as well as Cav-1, IR, PKB/AKT-2 and Glut-4 expression and activation by real time RT-PCR and western blot. Baseline and insulin-induced glucose uptake was measured by the 2-[C14]-deoxyglucose uptake assay. Results: TNF-α slowed down the differentiation program, hindering the expression of some insulin signaling intermediates without fully eliminating insulin-mediated glucose uptake. In mature adipocytes, TNF-α did not compromise lipidstorage capacity, but downregulated the expression of the insulin signaling intermediates, totally blocking insulin-mediated glucose uptake. Insulin sensitivity correlated with the level of activated phospho-Cav-1 in both situations, strongly suggesting the direct contribution of Cav-1 to the maintenance of this physiological response. Conclusion: Cav-1 activation by phosphorylation seems to be essential for the maintenance of an active and insulin-sensitive glucose uptake. | es_ES |
dc.editorial.note | Creative Commons Attribution-NonCommercial 3.0 Unported license (CC BY-NC) | es_ES |
dc.identifier.citation | Palacios-Ortega S, Varela-Guruceaga M, Algarabel M, Milagro FI, Martínez JA, de Miguel C. Effect of TNF-Alpha on caveolin-1 expression and insulin signaling during adipocyte differentiation and in mature adipocytes. Cell Physiol Biochem. 2015 Jul 6;36(4):1499-1516 | es_ES |
dc.identifier.doi | http://dx.doi.org/10.1159/000430314 | es_ES |
dc.identifier.issn | 1015-8987 | |
dc.identifier.uri | https://hdl.handle.net/10171/39073 | |
dc.language.iso | eng | es_ES |
dc.publisher | Karger | es_ES |
dc.relation | CIBERobn, Nutrigenio project (MINECO, Spain) and Linea Especial “Nutrition, Obesity and Health” of the University of Navarra (LE/97) | |
dc.relation.center | Facultad de Farmacia y Nutrición | |
dc.relation.department | Departamento de Ciencias de la Alimentación, Fisiología y Toxicología | |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Adipogenesis | es_ES |
dc.subject | Inflammation | es_ES |
dc.subject | Insulin receptor | es_ES |
dc.subject | Insulin-stimulated glucose uptake | es_ES |
dc.subject | DNA methylation | es_ES |
dc.subject | 3T3-L1 | es_ES |
dc.title | Effect of TNF-Alpha on caveolin-1 expression and insulin signaling during adipocyte differentiation and in mature adipocytes | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dspace.entity.type | Publication | es |
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