Effect of TNF-Alpha on caveolin-1 expression and insulin signaling during adipocyte differentiation and in mature adipocytes

dc.contributor.authorMartinez, J.A. (José Alfredo)
dc.contributor.authorAlgarabel, M. (Miriam)
dc.contributor.authorMiguel-Vázquez, C. (Carlos) de
dc.contributor.authorMilagro-Yoldi, F.I. (Fermín Ignacio)
dc.contributor.authorPalacios-Ortega, S. (Sara)
dc.contributor.authorVarela-Guruceaga, M. (Maider)
dc.date.accessioned2015-09-21T16:12:29Z
dc.date.available2015-09-21T16:12:29Z
dc.date.issued2015
dc.description.abstractBackground/Aims: Tumor necrosis factor-α (TNF-α)-mediated chronic low-grade inflammation of adipose tissue is associated with obesity and insulin resistance. Caveolin-1 (Cav-1) is the central component of adipocyte caveolae and has an essential role in the regulation of insulin signaling. The effects of TNF-α on Cav-1 expression and insulin signaling during adipocyte differentiation and in mature adipocytes were studied. Methods: 3T3-L1 cells were differentiated (21 days) in the presence TNF-α (10 ng/mL) and mature adipocytes were also treated with TNF-α for 48 hours. Cav-1 and insulin receptor (IR) gene methylation were determined as well as Cav-1, IR, PKB/AKT-2 and Glut-4 expression and activation by real time RT-PCR and western blot. Baseline and insulin-induced glucose uptake was measured by the 2-[C14]-deoxyglucose uptake assay. Results: TNF-α slowed down the differentiation program, hindering the expression of some insulin signaling intermediates without fully eliminating insulin-mediated glucose uptake. In mature adipocytes, TNF-α did not compromise lipidstorage capacity, but downregulated the expression of the insulin signaling intermediates, totally blocking insulin-mediated glucose uptake. Insulin sensitivity correlated with the level of activated phospho-Cav-1 in both situations, strongly suggesting the direct contribution of Cav-1 to the maintenance of this physiological response. Conclusion: Cav-1 activation by phosphorylation seems to be essential for the maintenance of an active and insulin-sensitive glucose uptake.es_ES
dc.editorial.noteCreative Commons Attribution-NonCommercial 3.0 Unported license (CC BY-NC)es_ES
dc.identifier.citationPalacios-Ortega S, Varela-Guruceaga M, Algarabel M, Milagro FI, Martínez JA, de Miguel C. Effect of TNF-Alpha on caveolin-1 expression and insulin signaling during adipocyte differentiation and in mature adipocytes. Cell Physiol Biochem. 2015 Jul 6;36(4):1499-1516es_ES
dc.identifier.doihttp://dx.doi.org/10.1159/000430314es_ES
dc.identifier.issn1015-8987
dc.identifier.urihttps://hdl.handle.net/10171/39073
dc.language.isoenges_ES
dc.publisherKargeres_ES
dc.relationCIBERobn, Nutrigenio project (MINECO, Spain) and Linea Especial “Nutrition, Obesity and Health” of the University of Navarra (LE/97)
dc.relation.centerFacultad de Farmacia y Nutrición
dc.relation.departmentDepartamento de Ciencias de la Alimentación, Fisiología y Toxicología
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectAdipogenesises_ES
dc.subjectInflammationes_ES
dc.subjectInsulin receptores_ES
dc.subjectInsulin-stimulated glucose uptakees_ES
dc.subjectDNA methylationes_ES
dc.subject3T3-L1es_ES
dc.titleEffect of TNF-Alpha on caveolin-1 expression and insulin signaling during adipocyte differentiation and in mature adipocyteses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
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