Insertion (22;9)(q11;q34q21) in a patient with chronic myeloid leukemia characterized by fluorescence in situ hybridization

Martin-Subero, J.I. (Jose Ignacio); Lahortiga, I. (Idoya); Gomez, E. (Emilio); Ferreira, C. (C.); Larrayoz, M.J. (María J.); Odero, M.D. (Maria Dolores); García-Delgado, M. (Marina); Novo-Villaverde, F. J. (Francisco Javier); Giraldo, P. (P.); Calasanz-Abinzano, M.J. (Maria Jose)

Keywords:

Chromosome Inversion
Chromosomes, Human, Pair 22/ultrastructure
Chromosomes, Human, Pair 9/ultrastructure
Fusion Proteins, bcr-abl/genetics
Leukemia, Myelogenous, Chronic, BCR-ABL Positive/genetics
Translocation, Genetic
Tumor Markers, Biological/genetics

Issue Date:

2001

Publisher:

Elsevier

Publisher Version:

http://www.sciencedirect.com/science/article/pii/S0165460800003708

ISSN:

1873-4456

Citation:

Martin-Subero JI, Lahortiga I, Gomez E, Ferreira C, Larrayoz MJ, Odero MD, et al. Insertion (22;9)(q11;q34q21) in a patient with chronic myeloid leukemia characterized by fluorescence in situ hybridization. Cancer Genet Cytogenet 2001 Mar;125(2):167-170.

Abstract

An unusual cytogenetic rearrangement, described as ins(22;9)(q11;q34q21), was detected in a 49-year-old male patient diagnosed with chronic myeloid leukemia (CML). Reverse transcriptase polymerase chain reaction (RT-PCR) revealed a b3a2 fusion transcript. In order to confirm the cytogenetic findings and fully characterize the inverted insertion, we performed fluorescence in situ hybridization (FISH) assays using locus-specific and whole chromosome painting probes. Our FISH analysis showed the presence of the BCR/ABL fusion gene, verified the insertion and determined that the breakpoint on chromosome 22 where the insertion took place was located proximal to the BCR gene and distal to the TUPLE1 gene on 22q11.

URI:

https://hdl.handle.net/10171/19673

Appears in Collections:

DA - Ciencias - Bioquímica y Genética - Artículos de revista
DA - CIMA - Hemato-oncología - Leucemia aguda - Artículos de Revista

Files in This Item: