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dc.creatorGamazo, C. (Carlos)-
dc.creatorWinter, A.J. (A. J.)-
dc.creatorMoriyon, I. (Ignacio)-
dc.creatorRiezu-Boj, J.I. (José Ignacio)-
dc.creatorBlasco, J.M. (J. M.)-
dc.creatorDiaz, R. (Ramón)-
dc.identifier.citationGamazo C, Winter AJ, Moriyon I, Riezu-Boj JI, Blasco JM, Diaz R. Comparative analyses of proteins extracted by hot saline or released spontaneously into outer membrane blebs from field strains of Brucella ovis and Brucella melitensis. Infect Immun 1989 May;57(5):1419-1426.es_ES
dc.description.abstractSheep infected with Brucella ovis produce antibody responses to the rough lipopolysaccharide and to proteins present in hot saline (HS) extracts of B. ovis (J. I. Riezu-Boj, I. Moriyón, J. M. Blasco, C. M. Marín, and R. Díaz, J. Clin. Microbiol. 23:938-942, 1986). The distribution and antigenic relatedness of proteins in HS extracts and in outer membrane blebs were established by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting for 41 strains of B. ovis and 26 strains of Brucella melitensis of diverse geographic origin. Five major groups of proteins were identified in HS extracts of B. ovis that had been freed of rough lipopolysaccharide: proteins of 43 kilodaltons (kDa), group A (25.5 to 32.0 kDa), group B (21.5 to 22.5 kDa); group C (18.0 to 19.5 kDa), and group D (13.0 to 15.5 kDa). Group A, B, C, and D proteins were also present in blebs. The profiles of proteins in HS extracts or blebs from strains of both Brucella species were very similar. Cross-reactions were demonstrated among HS extracts and blebs of all strains tested in immunoblots performed with an antiserum against the HS extract of a reference strain of B. ovis. Evidence was also provided of an antigenic relationship between group 3 proteins of the outer membrane and some of the proteins in groups A, B, and C. The conservation of these antigens and their immunogenicity in infected animals provide promise that they may serve as components of an effective subcellular vaccine for ovine brucellosis.es_ES
dc.publisherAmerican Society for Microbiologyes_ES
dc.subjectAntigens, Bacterial/analysises_ES
dc.subjectBacterial Outer Membrane Proteins/analysises_ES
dc.titleComparative analyses of proteins extracted by hot saline or released spontaneously into outer membrane blebs from field strains of Brucella ovis and Brucella melitensises_ES

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