Materias Investigacion::Ciencias de la Salud::Microbiología y biología molecular Materias Investigacion::Ciencias de la Salud::Oncología Mesenchymal stem cells Adipose tissue Human iPSC line Stem cell
An open access article under the CC BY-NC-ND license
Zapata-Linares NM, Rodríguez S, Mazo M, Abizanda G, Andreu EJ, Barajas M, et al. Generation and characterization of human iPSC line generated from mesenchymal stem cells derived from adipose tissue. Stem Cell Res 2016;16(1):20–23
In this work, mesenchymal stem cells derived from adipose tissue (ADSCs) were used for the generation of the human-induced pluripotent stem cell line G15.AO. Cell reprogramming was performed using retroviral vectors containing the Yamanaka factors, and the generated G15.AO hiPSC line showed normal karyotype, silencing of the exogenous reprogramming factors, induction of the typical pluripotency-associated markers, alkaline phosphatase enzymatic activity, and in vivo and in vitro differentiation ability to the three germ layers.