Full metadata record
DC Field | Value | Language |
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dc.creator | Naldaiz-Gastesi, N. (Neia) | - |
dc.creator | Goicoechea, M. (María) | - |
dc.creator | Aragón, I.M. (Isabel María) | - |
dc.creator | Pérez-López, V. (Virginia) | - |
dc.creator | Fuertes-Alvarez, S. (Sandra) | - |
dc.creator | Herrera-Imbroda, B. (Bernardo) | - |
dc.creator | Lopez-de-Munain, A. (Adolfo) | - |
dc.creator | Luna-Diaz, R. (Resi) de | - |
dc.creator | Baptista, P.M. (Pedro M.) | - |
dc.creator | Fernández, M.A. (M. Alejandro) | - |
dc.creator | Lara, M.F. (María Fernanda) | - |
dc.creator | Izeta, A. (Ander) | - |
dc.date.accessioned | 2021-10-01T16:01:07Z | - |
dc.date.available | 2021-10-01T16:01:07Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Naldaiz-Gastesi, N. (Neia); Goicoechea, M. (María); Aragón, I.M. (Isabel María); et al. "Isolation and characterization of myogenic precursor cells from human cremaster muscle". Scientific Reports. 9 (3454), 2019, 1 - 15 | es |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.other | PMID: 30837559 | - |
dc.identifier.uri | https://hdl.handle.net/10171/62137 | - |
dc.description.abstract | Human myogenic precursor cells have been isolated and expanded from a number of skeletal muscles, but alternative donor biopsy sites must be sought after in diseases where muscle damage is widespread. Biopsy sites must be relatively accessible, and the biopsied muscle dispensable. Here, we aimed to histologically characterize the cremaster muscle with regard number of satellite cells and regenerative fibres, and to isolate and characterize human cremaster muscle-derived stem/precursor cells in adult male donors with the objective of characterizing this muscle as a novel source of myogenic precursor cells. Cremaster muscle biopsies (or adjacent non-muscle tissue for negative controls; N = 19) were taken from male patients undergoing routine surgery for urogenital pathology. Myosphere cultures were derived and tested for their in vitro and in vivo myogenic differentiation and muscle regeneration capacities. Cremaster-derived myogenic precursor cells were maintained by myosphere culture and efficiently differentiated to myotubes in adhesion culture. Upon transplantation to an immunocompromised mouse model of cardiotoxin-induced acute muscle damage, human cremaster-derived myogenic precursor cells survived to the transplants and contributed to muscle regeneration. These precursors are a good candidate for cell therapy approaches of skeletal muscle. Due to their location and developmental origin, we propose that they might be best suited for regeneration of the rhabdosphincter in patients undergoing stress urinary incontinence after radical prostatectomy. | es_ES |
dc.description.sponsorship | Tis work was supported by grants from Ministerio de Economía y Competitividad (RTC-2015-3750-1) and Instituto de Salud Carlos III (PI13/02172, PI16/01430) to A.I., co-funded by the European Union (ERDF/ESF, ‘Investing in your future’). N.N.-G. received a studentship from the Department of Education, University and Research of the Basque Government (PRE2013- 1-1168). A.L.M. was funded by grants from FIS (PI17/01841 and PI14/00436), CIBERNED and the Basque Government (2015/11038, RIS3 2017222021 and BIO16/ER/022). M.F.L.-C. was supported by the Servicio Andaluz de Salud from the Consejería de Salud de la Junta de Andalucía, grant PI 0222-2014, co-funded by the European Union (ERDF/ESF). I.M.A was funded by grants from Ministerio de Economia y Competitividad (PEJ2014-P-01215 and FJCI-2016-28121). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Springer Science and Business Media LLC | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Materias Investigacion::Ingeniería::Biotecnología | es_ES |
dc.title | Isolation and characterization of myogenic precursor cells from human cremaster muscle | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.description.note | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made | es_ES |
dc.identifier.doi | 10.1038/s41598-019-40042-6 | - |
dadun.citation.endingPage | 15 | es_ES |
dadun.citation.number | 3454 | es_ES |
dadun.citation.publicationName | Scientific Reports | es_ES |
dadun.citation.startingPage | 1 | es_ES |
dadun.citation.volume | 9 | es_ES |
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