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dc.creatorLuquin, E. (Esther)-
dc.creatorPaternain, B. (B.)-
dc.creatorZugasti, I. (Inés)-
dc.creatorSantomá, C. (Carmen)-
dc.creatorMengual, E. (Elisa)-
dc.date.accessioned2022-04-05T09:38:37Z-
dc.date.available2022-04-05T09:38:37Z-
dc.date.issued2021-
dc.identifier.citationLuquin, E. (Esther); Paternain, B. (B.); Zugasti, I. (Inés); et al. "Stereological estimations and neurochemical characterization of neurons expressing GABAA and GABAB receptors in the rat pedunculopontine and laterodorsal tegmental nuclei". Brain Structure and Function. (227), 2021, 89 - 110es_ES
dc.identifier.issn1863-2661-
dc.identifier.urihttps://hdl.handle.net/10171/63343-
dc.description.abstractTo better understand GABAergic transmission at two targets of basal ganglia downstream projections, the pedunculopontine (PPN) and laterodorsal (LDT) tegmental nuclei, the anatomical localization of GABAA and GABAB receptors was investigated in both nuclei. Specifically, the total number of neurons expressing the GABAA receptor γ2 subunit (GABAAR γ2) and the GABAB receptor R2 subunit (GABAB R2) in PPN and LDT was estimated using stereological methods, and the neurochemical phenotype of cells expressing each subunit was also determined. The mean number of non-cholinergic cells expressing GABAAR γ2 was 9850 ± 1856 in the PPN and 8285 ± 962 in the LDT, whereas those expressing GABAB R2 were 7310 ± 1970 and 9170 ± 1900 in the PPN and LDT, respectively. In addition, all cholinergic neurons in both nuclei co-expressed GABAAR γ2 and 95–98% of them co-expressed GABAB R2. Triple labeling using in situ hybridization revealed that 77% of GAD67 mRNA-positive cells in the PPT and 49% in the LDT expressed GABAAR γ2, while 90% (PPN) and 65% (LDT) of Vglut2 mRNA-positive cells also expressed GABAAR γ2. In contrast, a similar proportion (~2/3) of glutamatergic and GABAergic cells co-expressed GABAB R2 in both nuclei. The heterogeneous distribution of GABAAR and GABABR among non-cholinergic cells in PPN and LDT may give rise to physiological differences within each neurochemical subpopulation. In addition, the dissimilar proportion of GABAAR γ2-expressing glutamatergic and GABAergic neurons in the PPN and LDT may contribute to some of the functional differences found between the two nuclei.es_ES
dc.description.sponsorshipOpen Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. The present work was funded by Plan de Investigación de la Universidad de Navarra (PIUNA, 2014).es_ES
dc.language.isoenges_ES
dc.publisherSpringeres_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectBasal gangliaes_ES
dc.subjectGAD67es_ES
dc.subjectVglut2es_ES
dc.subjectIn situ hybridizationes_ES
dc.subjectGaites_ES
dc.subjectRewardes_ES
dc.titleStereological estimations and neurochemical characterization of neurons expressing GABAA and GABAB receptors in the rat pedunculopontine and laterodorsal tegmental nucleies_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.noteThis article is licensed under a Creative Commons Attribution 4.0 International Licensees_ES
dc.identifier.doi10.1007/s00429-021-02375-9-
dadun.citation.endingPage110es_ES
dadun.citation.number227es_ES
dadun.citation.publicationNameBrain Structure and Functiones_ES
dadun.citation.startingPage89es_ES

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