Integration of CRISPR-Cas9, shRNA with other genomic data provides reliable predicions of gene essentiality

Abstract

Recent genome-wide loss-of-function screening studies have provided an unprecedented amount of information to perform functional analysis of the genome. Two main strategies have been followed to perform these experiments: shRNA and, more recently, CRISPR-Cas9. Both technologies have shown their ability to knock down genes and, in the case of CRISPR-Cas9, the ability to create new cell-line strands with different phenotypes