Full metadata record
DC Field | Value | Language |
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dc.creator | Lütjohann, D. (Dieter) | - |
dc.creator | Björkhem, I. (Ingemar) | - |
dc.creator | Friedrichs, S. (Silvia) | - |
dc.creator | Kerksiek, A. (Anja) | - |
dc.creator | Lövgren-Sandblom, A. (Anita) | - |
dc.creator | Geilenkeuser, W.J. (Wolf Jochen) | - |
dc.creator | Ahrends, R. (Robert) | - |
dc.creator | Andrade, I. (Isabel) | - |
dc.creator | Ansorena-Artieda, D. (Diana) | - |
dc.creator | Astiasarán, I. (Iciar) | - |
dc.creator | Baila-Rueda, L. (Lucía) | - |
dc.creator | Barriuso, B. (Blanca) | - |
dc.creator | Becker, S. (Susen) | - |
dc.creator | Bretillon, L. (Lionel) | - |
dc.creator | Browne, R.W. (Richard W.) | - |
dc.creator | Caccia, C. (Claudio) | - |
dc.creator | Ceglarek, U. (Uta) | - |
dc.creator | Cenarro, A. (Ana) | - |
dc.creator | Crick, P.J. (Peter J.) | - |
dc.creator | Fauler, G. (Günter) | - |
dc.creator | Garcia-Llatas, G. (Guadalupe) | - |
dc.creator | Gray, R. (Robert) | - |
dc.creator | Griffiths, W.J. (William J.) | - |
dc.creator | Gylling, H. (Helena) | - |
dc.creator | Harding, S. (Scott) | - |
dc.creator | Helmschrodt, C. (Christin) | - |
dc.creator | Iuliano, L. (Luigi) | - |
dc.creator | Janssen, H.G. (Hans Gerd) | - |
dc.creator | Jones, P. (Peter) | - |
dc.creator | Kaipiainen, L. (Leena) | - |
dc.creator | Kannenberg, F. (Frank) | - |
dc.creator | Lagarda, M.J. (María Jesús) | - |
dc.creator | Leoni, V. (Valerio) | - |
dc.creator | Lottenberg, A.M. (Ana María) | - |
dc.creator | Myrie, S.B. (Semone B.) | - |
dc.creator | MacKay, D.S. (Dylan S.) | - |
dc.creator | Matysik, S. (Silke) | - |
dc.creator | McDonald, J. (Jeff) | - |
dc.creator | Menendez-Carreño, M. (María) | - |
dc.creator | Nunes, V.S. (Valeria Sutti) | - |
dc.creator | Ostlund, R.E. (Richard E.) | - |
dc.creator | Polisecki, E. (Eliana) | - |
dc.creator | Ramos, F. (Fernando) | - |
dc.creator | Rideout, T.C. (Todd C.) | - |
dc.creator | Schaefer, E.J. (Ernst J.) | - |
dc.creator | Schmitz, G. (Gerd) | - |
dc.creator | Wang, Y. (Yuqin) | - |
dc.creator | Zerbinati, C. (Chiara) | - |
dc.creator | Diczfalusy, U. (Ulf) | - |
dc.creator | Schött, H.F. (Hans Frieder) | - |
dc.date.accessioned | 2019-06-11T10:00:03Z | - |
dc.date.available | 2019-06-11T10:00:03Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Lütjohann, D. (Dieter); Björkhem, I. (Ingemar); Friedrichs, S. (Silvia); et al. "First international descriptive and interventional survey for cholesterol and non-cholesterol sterol determination by gas- and liquid- chromatography–Urgent need for harmonisation of analytical methods". Journal of Steroid Biochemistry and Molecular Biology. 190, 2019, 115 - 125 | es_ES |
dc.identifier.issn | 0960-0760 | - |
dc.identifier.uri | https://hdl.handle.net/10171/57773 | - |
dc.description.abstract | Serum concentrations of lathosterol, the plant sterols campesterol and sitosterol and the cholesterol metabolite 5α-cholestanol are widely used as surrogate markers of cholesterol synthesis and absorption, respectively. Increasing numbers of laboratories utilize a broad spectrum of well-established and recently developed methods for the determination of cholesterol and non-cholesterol sterols (NCS). In order to evaluate the quality of these measurements and to identify possible sources of analytical errors our group initiated the first international survey for cholesterol and NCS. The cholesterol and NCS survey was structured as a two-part survey which took place in the years 2013 and 2014. The first survey part was designed as descriptive, providing information about the variation of reported results from different laboratories. A set of two lyophilized pooled sera (A and B) was sent to twenty laboratories specialized in chromatographic lipid analysis. The different sterols were quantified either by gas chromatography-flame ionization detection, gas chromatography- or liquid chromatography-mass selective detection. The participants were requested to determine cholesterol and NCS concentrations in the provided samples as part of their normal laboratory routine. The second part was designed as interventional survey. Twenty-two laboratories agreed to participate and received again two different lyophilized pooled sera (C and D). In contrast to the first international survey, each participant received standard stock solutions with defined concentrations of cholesterol and NCS. The participants were requested to use diluted calibration solutions from the provided standard stock solutions for quantification of cholesterol and NCS. In both surveys, each laboratory used its own internal standard (5α-cholestane, epicoprostanol or deuterium labelled sterols). Main outcome of the survey was, that unacceptably high interlaboratory variations for cholesterol and NCS concentrations are reported, even when the individual laboratories used the same calibration material. We discuss different sources of errors and recommend all laboratories analysing cholesterol and NCS to participate in regular quality control programs. | es_ES |
dc.description.sponsorship | We gratefully acknowledge funding for materials, statistics, and travel expenses to the Foundation for Pathobiochemistry and Molecular Diagnostics, German Society of Clinical Chemistry and Laboratory Medicine, Germany. The work in University of Valencia was financed by CICYT-FEDER (AGL2008−02591-C02-01) and MINECO-FEDER (AGL2012−39503-C02-01). The work at Universitario Miguel Servet, Zaragoza, Spain was funded by Fondo de Investigación Sanitaria (FIS; PI15/01983). Work in Swansea was supported by the UK Biotechnology and Biological Sciences Research Council (BBSRC, grant numbers BB/ I001735/1 and BB/L001942/1). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Surrogate marker | es_ES |
dc.subject | Cholesterol absorption | es_ES |
dc.subject | Cholesterol synthesis | es_ES |
dc.subject | Cholesterol balance | es_ES |
dc.subject | Atherosclerosis | es_ES |
dc.subject | Phytosterols | es_ES |
dc.title | First international descriptive and interventional survey for cholesterol and non-cholesterol sterol determination by gas- and liquid- chromatography–Urgent need for harmonisation of analytical methods | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.description.note | This is an open access article distributed under the Creative Commons: Atribution License (cc BY) | es_ES |
dc.identifier.doi | https://doi.org/10.1016/j.jsbmb.2019.03.025 | es_ES |
dc.identifier.doi | 10.1016/j.jsbmb.2019.03.025 | - |
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