Modulation of the fate of zein nanoparticles by their coating with a Gantrez® AN-thiamine polymer conjugate
Keywords: 
Zein
Nanoparticles
Thiamine
Oral delivery
Coating
Mucus permeating
Issue Date: 
2019
ISSN: 
Elsevier BV
Note: 
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).
Citation: 
Inchaurraga-Casadamon, L. (Laura); Martínez-López, A.L. (Ana L.); Abdulkarim, M. (Muthanna); et al. "Modulation of the fate of zein nanoparticles by their coating with a Gantrez® AN-thiamine polymer conjugate". International Journal of Pharmaceutics: X. 1, 2019, 100006
Abstract
The aim of this work was to evaluate the mucus-permeating properties of nanocarriers using zein nanoparticles (NPZ) coated with a Gantrez® AN-thiamine conjugate (GT). NPZ were coated by incubation at different GT-tozein ratios: 2.5% coating with GT (GT-NPZ1), 5% (GT-NPZ2) and 10% (GT-NPZ3). During the process, the GT conjugate formed a polymer layer around the surface of zein nanoparticles. For GT-NPZ2, the thickness of this corona was estimated between 15 and 20 nm. These nanocarriers displayed a more negative zeta potential than uncoated NPZ. The diffusivity of nanoparticles was evaluated in pig intestinal mucus by multiple particle tracking analysis. GT-NPZ2 displayed a 28-fold higher diffusion coefficient within the mucus layer than NPZ particles. These results align with in vivo biodistribution studies in which NPZ displayed a localisation restricted to the mucus layer, whereas GT-NPZ2 were capable of reaching the intestinal epithelium. The gastro-intestinal transit of mucoadhesive (NPZ) and mucus-permeating nanoparticles (GT-NPZ2) was also found to be different. Thus, mucoadhesive nanoparticles displayed a significant accumulation in the stomach of animals, whereas mucus-penetrating nanoparticles appeared to exit the stomach more rapidly to access the small intestine of animals

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