An engineered periosteum for efficient delivery of rhBMP-2 and mesenchymal progenitor cells during bone regeneration
Keywords: 
Área de Biomedicina
Induced membrane technique.
Growth-factor delivery.
Stem-cells.
Morphogenetic protein-2.
Biomimetic periosteum.
Defects.
BMP-2
Rat.
Model
Differentiation
Issue Date: 
2023
ISSN: 
2057-3995
Editorial note: 
This article is licensed under a Creative Commons Attribution 4.0 International License.
Citation: 
Romero-Torrecilla, J. (Juan Antonio); Lamo de Espinosa-Vázquez de Sola, J. (José María); Ripalda-Cemboráin, P. (Purificación); et al. "An engineered periosteum for efficient delivery of rhBMP-2 and mesenchymal progenitor cells during bone regeneration". NPJ regenerativa medicine. 8 (1), 2023, 54
Abstract
During bone regeneration, the periosteum acts as a carrier for key regenerative cues, delivering osteochondroprogenitor cells and crucial growth factors to the injured bone. We developed a biocompatible, 3D polycaprolactone (PCL) melt electro-written membrane to act as a mimetic periosteum. Poly (ethyl acrylate) coating of the PCL membrane allowed functionalization, mediated by fibronectin and low dose recombinant human BMP-2 (rhBMP-2) (10-25 mu g/ml), resulting in efficient, sustained osteoinduction in vitro. In vivo, rhBMP-2 functionalized mimetic periosteum demonstrated regenerative potential in the treatment of rat critical-size femoral defects with highly efficient healing and functional recovery (80%-93%). Mimetic periosteum has also proven to be efficient for cell delivery, as observed through the migration of transplanted periosteum-derived mesenchymal cells to the bone defect and their survival. Ultimately, mimetic periosteum demonstrated its ability to deliver key stem cells and morphogens to an injured site, exposing a therapeutic and translational potential in vivo when combined with unprecedentedly low rhBMP-2 doses.

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